Scholarlink

<p>Vesicle-associated viruses, or viral vesicles, are groups of virions packaged within or bound to the surface of small membrane sacs released from host cells. This structure has recently been identified as a common form of certain viral pathogens in host bodies and excreta and is known to enhance viral infectivity, virulence and persistence, raising health concerns if discharged into aquatic environments. However, the presence of vesicle-associated viruses in environments remains unexplored. Here we show that vesicle-associated viruses are abundant and diverse in real wastewater. Using a highly selective immunomagnetic method, we successfully isolated viral vesicles from municipal and hospital wastewater. Quantitative PCR revealed that a substantial fraction of human noroviruses in wastewater were vesicle-associated, accounting for 17% of norovirus GI and 45% of norovirus GII on average. Metagenomic analysis further showed that wastewater vesicles carry diverse bacteriophages as well as human, animal and plant viruses. These findings highlight the need to consider vesicle-associated viruses in wastewater treatment and public health protection.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Discovery of emerging vesicle-associated viruses in wastewater and implications for engineering interventions

  • 期刊论文

  • 发表日期：

    2026年3月2日

查看全文
  • Yuepeng Sun,
  • Hongyue Zhang,
  • Minghao Han,
  • Yiwen Zhu,
  • Xiaonan Tang,
  • Huiyun Wu,
  • Tiong Gim Aw,
  • Nihal Altan-Bonnet,
  • Joan B. Rose,
  • Danmeng Shuai,
  • Mahmud Syed,
  • Yun Shen

摘要

Vesicle-associated viruses, or viral vesicles, are groups of virions packaged within or bound to the surface of small membrane sacs released from host cells. This structure has recently been identified as a common form of certain viral pathogens in host bodies and excreta and is known to enhance viral infectivity, virulence and persistence, raising health concerns if discharged into aquatic environments. However, the presence of vesicle-associated viruses in environments remains unexplored. Here we show that vesicle-associated viruses are abundant and diverse in real wastewater. Using a highly selective immunomagnetic method, we successfully isolated viral vesicles from municipal and hospital wastewater. Quantitative PCR revealed that a substantial fraction of human noroviruses in wastewater were vesicle-associated, accounting for 17% of norovirus GI and 45% of norovirus GII on average. Metagenomic analysis further showed that wastewater vesicles carry diverse bacteriophages as well as human, animal and plant viruses. These findings highlight the need to consider vesicle-associated viruses in wastewater treatment and public health protection.