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<p>Clinical studies routinely show that individuals suffer from vitamin D deficiency, which can result in health complications that include cardiovascular disease, autoimmune disorders, neurodegenerative diseases, and different skeletal deformities. Given its integral role in homeostasis and connection to many pathologies, early diagnosis of vitamin D deficiency is crucial. However, monitoring vitamin D levels is challenging, particularly in remote regions, due to the cost, time, and complexity of existing methods. Here, we develop an electrochemical biosensor for vitamin D based on antibody-functionalized MXenes, offering clinically relevant sensitivity, specificity, and amenability for point-of-care testing. Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene nanosheets are amine-functionalized by electrostatically-driven modification with polyethylenimine, whose functionalities are then used for covalent conjugation of anti-vitamin D antibodies via glutaraldehyde chemistry. This platform achieves a detection limit of 1 pg mL<sup>−1</sup> with a dynamic range (0.1–500 ng mL<sup>−1</sup>) that covers clinically relevant deficiency, insufficiency, sufficiency, and toxicity.</p>

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Antibody-functionalized MXene-based electrochemical biosensor for point-of-care detection of vitamin D deficiency

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年2月17日

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  • Sharat Chandra Barman,
  • Yuming Jin,
  • Jehad K. El-Demellawi,
  • Simil Thomas,
  • Nimer Wehbe,
  • Yongjiu Lei,
  • Mrinal Kanti Hota,
  • Xiangming Xu,
  • Erol A. Hasan,
  • Omar F. Mohammed,
  • Osman M. Bakr,
  • Dana Alsulaiman,
  • Husam N. Alshareef

摘要

Clinical studies routinely show that individuals suffer from vitamin D deficiency, which can result in health complications that include cardiovascular disease, autoimmune disorders, neurodegenerative diseases, and different skeletal deformities. Given its integral role in homeostasis and connection to many pathologies, early diagnosis of vitamin D deficiency is crucial. However, monitoring vitamin D levels is challenging, particularly in remote regions, due to the cost, time, and complexity of existing methods. Here, we develop an electrochemical biosensor for vitamin D based on antibody-functionalized MXenes, offering clinically relevant sensitivity, specificity, and amenability for point-of-care testing. Ti3C2Tx MXene nanosheets are amine-functionalized by electrostatically-driven modification with polyethylenimine, whose functionalities are then used for covalent conjugation of anti-vitamin D antibodies via glutaraldehyde chemistry. This platform achieves a detection limit of 1 pg mL−1 with a dynamic range (0.1–500 ng mL−1) that covers clinically relevant deficiency, insufficiency, sufficiency, and toxicity.