Scholarlink

<p><i>Wolbachia</i> is a widespread endosymbiont and a promising tool for pest control due to its ability to manipulate arthropod reproduction. However, how <i>Wolbachia</i> shapes host microRNA regulation remains poorly understood. Here, we profile the <i>Wolbachia</i>-driven host miRNAs using small RNA-seq in spider mite <i>Tetranychus truncatus</i> to uncover their roles in reproduction and symbiosis. Enrichment analyses of predicted miRNA targets suggest that <i>Wolbachia</i>-driven miRNAs may be involved in <i>Wolbachia</i>–host interactions. Functional assays show that several <i>Wolbachia</i>-driven miRNAs influence host fecundity, with some also affecting <i>Wolbachia</i> density. Inhibition of novel-33 or novel-40 reduces host fecundity and is accompanied by reduced <i>Wolbachia</i> density, whereas inhibition of novel-65 reduces fecundity without altering density. These findings indicate that specific miRNAs contribute to the regulation of reproduction and <i>Wolbachia</i> density in <i>T. truncatus</i>, highlighting host miRNAs regulated by <i>Wolbachia</i> as a mechanism of host manipulation and offering opportunities for <i>Wolbachia</i>-based pest management.</p><p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Wolbachia-driven host miRNAs mediate arthropod reproduction in a Wolbachia density-dependent manner

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月17日

查看全文
  • Yi-Yin Zhang,
  • Xin-Yu Shen,
  • Xiao-Hui Xiong,
  • Dian-Shu Zhao,
  • Xiao-Yue Hong

摘要

Wolbachia is a widespread endosymbiont and a promising tool for pest control due to its ability to manipulate arthropod reproduction. However, how Wolbachia shapes host microRNA regulation remains poorly understood. Here, we profile the Wolbachia-driven host miRNAs using small RNA-seq in spider mite Tetranychus truncatus to uncover their roles in reproduction and symbiosis. Enrichment analyses of predicted miRNA targets suggest that Wolbachia-driven miRNAs may be involved in Wolbachia–host interactions. Functional assays show that several Wolbachia-driven miRNAs influence host fecundity, with some also affecting Wolbachia density. Inhibition of novel-33 or novel-40 reduces host fecundity and is accompanied by reduced Wolbachia density, whereas inhibition of novel-65 reduces fecundity without altering density. These findings indicate that specific miRNAs contribute to the regulation of reproduction and Wolbachia density in T. truncatus, highlighting host miRNAs regulated by Wolbachia as a mechanism of host manipulation and offering opportunities for Wolbachia-based pest management.