Scholarlink

<p>Cryo-imaging in cellular biology provides the means to visualize the cellular interior at close-to-native conditions. A cornerstone in the field has been cryo-correlative light and electron microscopy (cryo-CLEM), with cryo-visible light fluorescent microscopy (cryo-VLFM) providing the specificity by tagging macromolecules or structures and cryo-electron tomography (cryo-ET) for the structural details at molecular level. The large resolution gap between these techniques, however, is limiting this correlative workflow as cryo-ET targets are often smaller than the resolution limit of cryo-VLFM. Here we introduce cryo-soft X-ray tomography (cryo-SXT) as an intermediate step that can compensate for the partial view caused by the lost cellular material due to FIB-milling and limited resolution of cryo-VLFM by providing invaluable cellular context information in 3D to the cryo-ET dataset within an integrated workflow. This work shows that X-ray and electron imaging are not mutually exclusive, creating opportunities for further correlative imaging strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Cryo-CLXEM introduces cryo-SXT to bridge the resolution gap in cryo-CLEM

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月25日

查看全文
  • Johannes Groen,
  • Anastasia Gazi,
  • Sergey Kapishnikov,
  • Anne Brelot,
  • Matthijn Vos,
  • Jost Enninga,
  • Eva Pereiro,
  • Anna Sartori-Rupp

摘要

Cryo-imaging in cellular biology provides the means to visualize the cellular interior at close-to-native conditions. A cornerstone in the field has been cryo-correlative light and electron microscopy (cryo-CLEM), with cryo-visible light fluorescent microscopy (cryo-VLFM) providing the specificity by tagging macromolecules or structures and cryo-electron tomography (cryo-ET) for the structural details at molecular level. The large resolution gap between these techniques, however, is limiting this correlative workflow as cryo-ET targets are often smaller than the resolution limit of cryo-VLFM. Here we introduce cryo-soft X-ray tomography (cryo-SXT) as an intermediate step that can compensate for the partial view caused by the lost cellular material due to FIB-milling and limited resolution of cryo-VLFM by providing invaluable cellular context information in 3D to the cryo-ET dataset within an integrated workflow. This work shows that X-ray and electron imaging are not mutually exclusive, creating opportunities for further correlative imaging strategies.