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<p>The gut microbiota plays a crucial role in mosquito biology and immunity, but the effects of <i>Wolbachia</i> on microbiota diversity remain unclear. In Brazil, <i>w</i>Mel-carrying <i>Aedes aegypti</i> were first deployed in 2015 to replace wild populations and block arbovirus transmission. We analyzed through 16S rRNA amplicon-sequencing (V3-V4 region) the midgut microbiota of field-caught <i>w</i>Mel- and <i>w</i>Mel + <i>Ae. aegypti</i> from areas of Rio de Janeiro with different <i>w</i>Mel invasion status. Overall, <i>w</i>Mel+ mosquitoes exhibited reduced bacterial diversity and altered microbiota composition. The interactions between bacterial taxa were also reduced in <i>w</i>Mel+ when compared to sympatric <i>w</i>Mel- mosquitoes from areas with partial <i>Wolbachia</i> introgression, suggesting a transitional microbial composition, whereas areas with full <i>Wolbachia</i> invasion displayed more homogeneous, densely connected bacterial communities. Although <i>Wolbachia</i> played a central role in microbial networks, its effects appeared contingent on other co-occurring taxa. Notably, certain bacteria such as <i>Acetobacteraceae</i> (<i>Asaia</i>), <i>Moraxellaceae</i> (<i>Acinetobacter</i>), and <i>Xanthomonadaceae</i> were associated with reduced <i>w</i>Mel abundance, potentially impacting its pathogen-blocking efficiency. Targeting these interactions may enhance the success of <i>Wolbachia</i>-based vector control strategies.</p>

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Midgut microbiota diversity and interactions shift when field Aedes aegypti mosquitoes carry the wMel strain of Wolbachia pipientis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月17日

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  • Jessica Corrêa-Antônio,
  • João M. C. Baltar,
  • Rafael Maciel-de-Freitas,
  • Mariana R. David,
  • Márcio G. Pavan

摘要

The gut microbiota plays a crucial role in mosquito biology and immunity, but the effects of Wolbachia on microbiota diversity remain unclear. In Brazil, wMel-carrying Aedes aegypti were first deployed in 2015 to replace wild populations and block arbovirus transmission. We analyzed through 16S rRNA amplicon-sequencing (V3-V4 region) the midgut microbiota of field-caught wMel- and wMel + Ae. aegypti from areas of Rio de Janeiro with different wMel invasion status. Overall, wMel+ mosquitoes exhibited reduced bacterial diversity and altered microbiota composition. The interactions between bacterial taxa were also reduced in wMel+ when compared to sympatric wMel- mosquitoes from areas with partial Wolbachia introgression, suggesting a transitional microbial composition, whereas areas with full Wolbachia invasion displayed more homogeneous, densely connected bacterial communities. Although Wolbachia played a central role in microbial networks, its effects appeared contingent on other co-occurring taxa. Notably, certain bacteria such as Acetobacteraceae (Asaia), Moraxellaceae (Acinetobacter), and Xanthomonadaceae were associated with reduced wMel abundance, potentially impacting its pathogen-blocking efficiency. Targeting these interactions may enhance the success of Wolbachia-based vector control strategies.