Scholarlink

<p>Increasing evidence shows that folate can regulate neural gene function through DNA modification, while regulatory roles of folate in neural RNA modification remain largely unknown. Here we show that folate treatment significantly increased <i>Drosophila</i> mRNA m<sup>6</sup>A levels. RNA methylation analysis indicates that carbon metabolism pathways and neural pathways are the mainly affected pathways by folate. Folate can increase mRNA m<sup>6</sup>A modification through carbon metabolic pathway in human cells, especially in neural cells. We also show that folate treatment can significantly increase the expression of m<sup>6</sup>A-related proteins (METTL3, METTL14) and neural mRNA m<sup>6</sup>A methylation levels in mice brain. Moreover, we find that folate-producing <i>Lactobacillus plantarum</i> can increase host mRNA m<sup>6</sup>A modification after colonization. In conclusion, we demonstrate that folate participates in mRNA m<sup>6</sup>A modification through carbon metabolic pathway in both <i>Drosophila</i> and mammals, and our results suggest that folate has a significant effect on neural-related genes and pathways.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transcriptome-wide regulation of folate on neural mRNA m6A methylome via carbon metabolism in Drosophila and mammals

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月4日

查看全文
  • Anrui Liu,
  • Zhengyi Huo,
  • Zihe Xu,
  • Sisi Liu,
  • Yuting Jiao,
  • Rui Li,
  • Yi Lu,
  • Meng Yang,
  • Jia Huang,
  • Chen Huang,
  • Xiaoyun Wang

摘要

Increasing evidence shows that folate can regulate neural gene function through DNA modification, while regulatory roles of folate in neural RNA modification remain largely unknown. Here we show that folate treatment significantly increased Drosophila mRNA m6A levels. RNA methylation analysis indicates that carbon metabolism pathways and neural pathways are the mainly affected pathways by folate. Folate can increase mRNA m6A modification through carbon metabolic pathway in human cells, especially in neural cells. We also show that folate treatment can significantly increase the expression of m6A-related proteins (METTL3, METTL14) and neural mRNA m6A methylation levels in mice brain. Moreover, we find that folate-producing Lactobacillus plantarum can increase host mRNA m6A modification after colonization. In conclusion, we demonstrate that folate participates in mRNA m6A modification through carbon metabolic pathway in both Drosophila and mammals, and our results suggest that folate has a significant effect on neural-related genes and pathways.