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<p>Developmental processes underlying the characteristic segmented body plans in arthropods vary widely. While <i>Drosophila</i> is well-studied, few other arthropod species offer platforms for comparable genomics at single-cell resolution. Here, we present high-quality quantitative data from single-nucleus RNA sequencing of spider <i>Parasteatoda tepidariorum</i> embryos at late stage 5 and stage 7, a critical period of emergence of segmental units along the anterior–posterior (AP) axis. Clustering analysis of the stage-7 dataset reconstructs an axial alignment of ectoderm cells, reflecting the differing cell states along the segmenting AP axis. This enables us to obtain genome-wide quantitative gene expression profiles along the reconstructed axis, which were used for unbiased and thorough molecular investigation of pattern elements employing statistical methods. Comprehensive gene-to-gene correlation analyses suggest distinct gene-regulatory interactions in different regions along the reconstructed axis. This study lays the foundation for exploring the origins of developmental diversity in the arthropod body plan.</p><p></p>

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Genome-wide quantitative dissection of an arthropod segmented body plan at single-cell resolution

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月11日

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  • Takanori Akaiwa,
  • Hiroki Oda,
  • Yasuko Akiyama-Oda

摘要

Developmental processes underlying the characteristic segmented body plans in arthropods vary widely. While Drosophila is well-studied, few other arthropod species offer platforms for comparable genomics at single-cell resolution. Here, we present high-quality quantitative data from single-nucleus RNA sequencing of spider Parasteatoda tepidariorum embryos at late stage 5 and stage 7, a critical period of emergence of segmental units along the anterior–posterior (AP) axis. Clustering analysis of the stage-7 dataset reconstructs an axial alignment of ectoderm cells, reflecting the differing cell states along the segmenting AP axis. This enables us to obtain genome-wide quantitative gene expression profiles along the reconstructed axis, which were used for unbiased and thorough molecular investigation of pattern elements employing statistical methods. Comprehensive gene-to-gene correlation analyses suggest distinct gene-regulatory interactions in different regions along the reconstructed axis. This study lays the foundation for exploring the origins of developmental diversity in the arthropod body plan.