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<p>Increasing evidence suggests vaginal dysbiosis is associated with persistent high-risk human papillomavirus (hrHPV) infection and cervical intraepithelial neoplasia (CIN) development. In this pilot longitudinal study, we investigate the potential of vaginal microbiome biomarkers to predict CIN3 development in hrHPV-positive (hrHPV+) women of reproductive age and assess loop electrosurgical excision procedure (LEEP) outcomes.</p><p>Fifty-nine non-menopausal women 20–53 years old, with normal cytology, were selected from the ARTISTIC trial and followed up twice over six years. Vaginal microbiome was analysed by 16S rRNA sequencing. HrHPV+ women with CIN3 showed a significant overrepresentation of <i>Sneathia amnii</i>, <i>Megasphaera genomosp., Peptostreptococcus anaerobius and Achromobacter spanius</i> (<i>p</i> &lt; 0.05). Successfully LEEP-treated hrHPV-negative women exhibited increased <i>Lactobacillus</i> species, especially <i>Lactobacillus gasseri</i>. Additionally, <i>Lactobacillus helveticus</i>, <i>suntoryeus</i> and <i>vaginalis</i> showed a potential protective role against CIN3 development.</p><p>These unique microbial biomarkers associated with CIN3 development and recovery following LEEP treatment bring new insights into the vaginal microbiome’s role on disease progression.</p>

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A longitudinal pilot study in pre-menopausal women links cervicovaginal microbiome to CIN3 progression and recovery

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月6日

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  • Cristiana Banila,
  • Efthymios Ladoukakis,
  • Dorota Scibior-Bentkowska,
  • Leandro Rodrigues Santiago,
  • Caroline Reuter,
  • Michelle Kleeman,
  • Belinda Nedjai

摘要

Increasing evidence suggests vaginal dysbiosis is associated with persistent high-risk human papillomavirus (hrHPV) infection and cervical intraepithelial neoplasia (CIN) development. In this pilot longitudinal study, we investigate the potential of vaginal microbiome biomarkers to predict CIN3 development in hrHPV-positive (hrHPV+) women of reproductive age and assess loop electrosurgical excision procedure (LEEP) outcomes.

Fifty-nine non-menopausal women 20–53 years old, with normal cytology, were selected from the ARTISTIC trial and followed up twice over six years. Vaginal microbiome was analysed by 16S rRNA sequencing. HrHPV+ women with CIN3 showed a significant overrepresentation of Sneathia amnii, Megasphaera genomosp., Peptostreptococcus anaerobius and Achromobacter spanius (p < 0.05). Successfully LEEP-treated hrHPV-negative women exhibited increased Lactobacillus species, especially Lactobacillus gasseri. Additionally, Lactobacillus helveticus, suntoryeus and vaginalis showed a potential protective role against CIN3 development.

These unique microbial biomarkers associated with CIN3 development and recovery following LEEP treatment bring new insights into the vaginal microbiome’s role on disease progression.