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<p>Optical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that the barcodes used for in situ genotyping are expressed from the chromosome. This has not previously been achieved in bacteria. Here we describe a method for in situ genotyping of libraries with genomic barcodes in <i>Escherichia coli</i>. The method is applied to measure the intracellular maturation time of 84 red fluorescent proteins.</p>

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Pooled optical screening in bacteria using chromosomally expressed barcodes

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月3日

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  • Ruben R. G. Soares,
  • Daniela A. García-Soriano,
  • Jimmy Larsson,
  • David Fange,
  • Dvir Schirman,
  • Marco Grillo,
  • Anna Knöppel,
  • Beer Chakra Sen,
  • Fabian Svahn,
  • Spartak Zikrin,
  • Michael Ratz,
  • Mats Nilsson,
  • Johan Elf

摘要

Optical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that the barcodes used for in situ genotyping are expressed from the chromosome. This has not previously been achieved in bacteria. Here we describe a method for in situ genotyping of libraries with genomic barcodes in Escherichia coli. The method is applied to measure the intracellular maturation time of 84 red fluorescent proteins.