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<p>Neuronal dense core vesicles (DCVs) store and release a diverse array of neuromodulators, trophic factors, and bioamines. The analysis of single DCVs has largely been possible only using electron microscopy, which makes understanding cargo segregation and DCV heterogeneity difficult. To address these limitations, we develop genetically encoded markers for DCVs that can be used in combination with standard immunohistochemistry and expansion microscopy to enable single-vesicle resolution with confocal microscopy in <i>Drosophila</i>.</p>

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Genetically-encoded markers for confocal visualization of single dense core vesicles

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月7日

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  • Junwei Yu,
  • Yunpeng Zhang,
  • Kelsey Clements,
  • Nannan Chen,
  • Leslie C. Griffith

摘要

Neuronal dense core vesicles (DCVs) store and release a diverse array of neuromodulators, trophic factors, and bioamines. The analysis of single DCVs has largely been possible only using electron microscopy, which makes understanding cargo segregation and DCV heterogeneity difficult. To address these limitations, we develop genetically encoded markers for DCVs that can be used in combination with standard immunohistochemistry and expansion microscopy to enable single-vesicle resolution with confocal microscopy in Drosophila.