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<p>Medium-chain alkanes have strong ecological impacts on marine ecosystems due to their persistence, toxicity, and ability to travel long distances. Microbial degradation is the dominant and ultimate removal process for n-alkanes in the deep ocean, where high hydrostatic pressure (HHP) regulates microbial activity. To gain insight into the impact of hydrostatic pressure (HP) on n-alkane degradation, we applied the deep-ocean experimental simulation to culture <i>Alcanivorax xenomutans</i> A28, a novel piezotolerant bacterium strain from trench sediment, with n-C<sub>16</sub> as the sole carbon source under different HPs (0.1, 40, and 80 MPa). Activity analysis demonstrated that HHP stimulated the n-C<sub>16</sub> complete mineralization ratio. Transcriptomic and metabolomic analyses showed that HHP induced the intracellular oxidative stress and accelerated the tricarboxylic acid (TCA) cycle. These results indicate a shift of n-alkanes biodegradation pattern regulated by HP, elucidating the fate and ecological risk of n-alkanes in the deep ocean.</p>

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High hydrostatic pressure stimulates n-C16 mineralization to CO2 by deep-ocean bacterium Alcanivorax xenomutans A28

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年2月16日

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  • Huaying Lin,
  • Yongxin Lv,
  • Yu Zhang

摘要

Medium-chain alkanes have strong ecological impacts on marine ecosystems due to their persistence, toxicity, and ability to travel long distances. Microbial degradation is the dominant and ultimate removal process for n-alkanes in the deep ocean, where high hydrostatic pressure (HHP) regulates microbial activity. To gain insight into the impact of hydrostatic pressure (HP) on n-alkane degradation, we applied the deep-ocean experimental simulation to culture Alcanivorax xenomutans A28, a novel piezotolerant bacterium strain from trench sediment, with n-C16 as the sole carbon source under different HPs (0.1, 40, and 80 MPa). Activity analysis demonstrated that HHP stimulated the n-C16 complete mineralization ratio. Transcriptomic and metabolomic analyses showed that HHP induced the intracellular oxidative stress and accelerated the tricarboxylic acid (TCA) cycle. These results indicate a shift of n-alkanes biodegradation pattern regulated by HP, elucidating the fate and ecological risk of n-alkanes in the deep ocean.