<p>The study on altermagnet is receiving extensive research efforts due to its directional-dependent spin-polarized band structure. Here, we investigate the multifunctional properties of the Janus Cr₂SeO monolayer system. The Cr₂SeO monolayer exhibits an in-plane magnetic anisotropy energy of -0.28 meV/cell and a Neel temperature of around 280 K, which is further enhanced to 315 K under 1% uniaxial compressive strain. The Janus Cr₂SeO exhibits spin band inversion with a large spin splitting of 0.38 eV at the Y and X high-symmetry points. Nonetheless, the valleys are degenerated due to the diagonal mirror symmetry. The uniaxial strain effectively breaks the diagonal mirror symmetry in the Janus Cr₂SeO monolayer, and the strain-induced valley polarization of up to 90 meV is achieved. Furthermore, the uniaxial strain tunes the band gap between 0.30 eV to 0.76 eV. Moreover, Cr₂SeO demonstrates ferroelectricity, with a total electric polarization of 5.34 pC/m, which increases to 5.52 pC/m under small uniaxial strain. Besides, we found a large vertical piezoelectric coefficient d₃₁ of 1.18 pm/V. Our study suggests that the Janus Cr₂SeO is a promising platform for Valleytronics and ferroelectric memory applications at room temperature.</p>