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<p>Bladder cancer is classified by depth of invasion into muscle-invasive bladder cancer (MIBC) and non-muscle-invasive bladder cancer (NMIBC). Because some NMIBC cases progress to MIBC, it is important to elucidate the mechanisms of invasion. Spatial transcriptomics is a powerful tool for characterizing the tumor microenvironment. We applied Visium to seven high-grade NMIBC tumors using en bloc transurethral resection of bladder tumor (ERBT) specimens that preserve three-dimensional tumor architecture. Malignant regions were divided into the tumor-upper and tumor-boundary regions, and seven cases were further stratified by pathological subgroup based on the muscularis mucosae. We identified 15 genes with boundary-enriched expression in the higher-risk subgroup. These genes were enriched for epithelial-mesenchymal transition and myogenesis features. To assess the generalizability of this finding, we analyzed public bulk RNA-seq datasets and found that higher expression of the 15-gene signature was associated with worse overall survival, suggesting potential utility for prognostic stratification and therapeutic decision-making.</p>

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Spatial transcriptomics of en bloc transurethral resection of bladder tumor specimens defines an invasive-front gene expression signature

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月14日

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  • Kentaro Yoshihara,
  • Fumihiko Urabe,
  • Miyaka Umemori,
  • Hironori Suzuki,
  • Kagenori Ito,
  • Takafumi Yanagisawa,
  • Shun Sato,
  • Keisuke Sekine,
  • Takahiro Kimura,
  • Yusuke Yamamoto

摘要

Bladder cancer is classified by depth of invasion into muscle-invasive bladder cancer (MIBC) and non-muscle-invasive bladder cancer (NMIBC). Because some NMIBC cases progress to MIBC, it is important to elucidate the mechanisms of invasion. Spatial transcriptomics is a powerful tool for characterizing the tumor microenvironment. We applied Visium to seven high-grade NMIBC tumors using en bloc transurethral resection of bladder tumor (ERBT) specimens that preserve three-dimensional tumor architecture. Malignant regions were divided into the tumor-upper and tumor-boundary regions, and seven cases were further stratified by pathological subgroup based on the muscularis mucosae. We identified 15 genes with boundary-enriched expression in the higher-risk subgroup. These genes were enriched for epithelial-mesenchymal transition and myogenesis features. To assess the generalizability of this finding, we analyzed public bulk RNA-seq datasets and found that higher expression of the 15-gene signature was associated with worse overall survival, suggesting potential utility for prognostic stratification and therapeutic decision-making.