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<p>The mandible is essential for facial structure and oral function. Jaw bone marrow mesenchymal stromal cells (JBMMSCs) exhibit self-renewal and multi-lineage potential for bone regeneration, but are compromised by post-injury inflammation, poor vascularization, and osteogenic microenvironment disruption. This study evaluated the effect of ultrashort wave (USW) therapy on enhancing JBMMSCs function and promoting mandibular repair. In a murine bone defect model, 3&#xa0;min USW treatment significantly improved bone regeneration (35.2 ± 3.8% increase in BV/TV, <i>P</i> &lt; 0.01) and induced a 42% reduction in fibrosis versus controls. In vitro, USW enhanced JBMMSCs proliferation (2.1-fold increase in Ki67 + cells) and osteogenesis (3.5-fold higher ALP activity; 4.2- and 3.8-fold upregulation of RUNX2 and OCN expression). RNA-seq identified 141 differentially expressed genes, with phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling as the most enriched pathway (FDR &lt; 0.01). Functional annotation of these transcriptomic alterations highlighted significant enrichment in cytokine responses and GTPase activity, suggesting that USW drives JBMMSC osteogenesis through the transcriptional modulation of the PI3K/Akt and JAK-STAT signaling networks These findings demonstrate USW therapy possibly promotes mandibular repair through JBMMSCs activity increase by regulating the gene expressions involved in the PI3K/Akt signaling pathway, with 3&#xa0;min exposure being optimal. This study offers both theoretical insights and clinical rationale for USW-based maxillofacial bone regeneration strategies.</p>

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Ultrashort wave therapy promotes the repair of jaw defect by regulating the function of jaw bone marrow mesenchymal stromal cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月31日

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  • Liu Liu,
  • Qian Zhao,
  • Jin Liu,
  • Huaqiang Ruan,
  • Zhangzhen Du,
  • Xu Liu,
  • Chuang Xu,
  • Ruoyan Zhang,
  • Haodi Li,
  • Chunkai Ma,
  • Feifei Tao,
  • Xiaoxia Jiang,
  • Lulu Xu

摘要

The mandible is essential for facial structure and oral function. Jaw bone marrow mesenchymal stromal cells (JBMMSCs) exhibit self-renewal and multi-lineage potential for bone regeneration, but are compromised by post-injury inflammation, poor vascularization, and osteogenic microenvironment disruption. This study evaluated the effect of ultrashort wave (USW) therapy on enhancing JBMMSCs function and promoting mandibular repair. In a murine bone defect model, 3 min USW treatment significantly improved bone regeneration (35.2 ± 3.8% increase in BV/TV, P < 0.01) and induced a 42% reduction in fibrosis versus controls. In vitro, USW enhanced JBMMSCs proliferation (2.1-fold increase in Ki67 + cells) and osteogenesis (3.5-fold higher ALP activity; 4.2- and 3.8-fold upregulation of RUNX2 and OCN expression). RNA-seq identified 141 differentially expressed genes, with phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling as the most enriched pathway (FDR < 0.01). Functional annotation of these transcriptomic alterations highlighted significant enrichment in cytokine responses and GTPase activity, suggesting that USW drives JBMMSC osteogenesis through the transcriptional modulation of the PI3K/Akt and JAK-STAT signaling networks These findings demonstrate USW therapy possibly promotes mandibular repair through JBMMSCs activity increase by regulating the gene expressions involved in the PI3K/Akt signaling pathway, with 3 min exposure being optimal. This study offers both theoretical insights and clinical rationale for USW-based maxillofacial bone regeneration strategies.