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<p>Previously, engineered strains lacking fatty acid synthase (<i>fas</i>) genes (<i>fas1</i> or <i>fas2</i>) were constructed in the oleaginous fungus <i>Mucor circinelloides</i> WJ11. The deletion of the <i>fas</i> gene not only increased lipid content but also enhanced the accumulation of β-carotene. This study aimed to investigate the impact of single-gene deletion of <i>fas1</i> and <i>fas2</i> on β-carotene synthesis in <i>M. circinelloides</i>. When compared with the control strain, the β-carotene contents in <i>fas</i>-knockout strains were increased by 1.6-fold for Mc-<i>fas1</i>Δ and 3.5-fold for Mc-<i>fas2</i>Δ, respectively. Meanwhile, the transcriptional levels of 3-hydroxy-3-methylglutaryl-CoA synthase (<i>hmgS</i>), 3-hydroxy-3-methylglutaryl-CoA reductase (<i>hmgR</i>), phytoene dehydrogenase (<i>carB</i>) and phytoene synthase/lycopene cyclase (<i>carRP</i>) involved in mevalonate pathway were significantly increased in <i>fas</i>-knockout strains. The yield of lipid and β-carotene in <i>fas</i>-knockout strains were significantly higher than those of the control strain. It was speculated that the deletion of the <i>fas</i> genes might initiate potent compensatory regulation to accelerate acetyl-CoA production, which supplies abundant precursors for lipid and carotenoid biosynthesis in <i>M. circinelloides</i>. To our knowledge, this is the first report uncovering the functional linkage between <i>fas</i> genes and β-carotene synthesis in this oleaginous fungus.</p>

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Improved β-carotene synthesis in fas-knockout Mucor circinelloides strains

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月28日

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  • Yao Zhang,
  • Yueping Yang,
  • Yan Sun,
  • Aichun Guan,
  • Qing Liu,
  • Yuanda Song

摘要

Previously, engineered strains lacking fatty acid synthase (fas) genes (fas1 or fas2) were constructed in the oleaginous fungus Mucor circinelloides WJ11. The deletion of the fas gene not only increased lipid content but also enhanced the accumulation of β-carotene. This study aimed to investigate the impact of single-gene deletion of fas1 and fas2 on β-carotene synthesis in M. circinelloides. When compared with the control strain, the β-carotene contents in fas-knockout strains were increased by 1.6-fold for Mc-fas1Δ and 3.5-fold for Mc-fas2Δ, respectively. Meanwhile, the transcriptional levels of 3-hydroxy-3-methylglutaryl-CoA synthase (hmgS), 3-hydroxy-3-methylglutaryl-CoA reductase (hmgR), phytoene dehydrogenase (carB) and phytoene synthase/lycopene cyclase (carRP) involved in mevalonate pathway were significantly increased in fas-knockout strains. The yield of lipid and β-carotene in fas-knockout strains were significantly higher than those of the control strain. It was speculated that the deletion of the fas genes might initiate potent compensatory regulation to accelerate acetyl-CoA production, which supplies abundant precursors for lipid and carotenoid biosynthesis in M. circinelloides. To our knowledge, this is the first report uncovering the functional linkage between fas genes and β-carotene synthesis in this oleaginous fungus.