Scholarlink

<p>Investigating the composition and diversity of <i>Anopheles</i> midgut microbiota is essential for understanding vector–pathogen interactions, insecticide resistance, and malaria transmission dynamics across diverse eco-epidemiological settings. This study characterized culture-dependent midgut microbiota of <i>Anopheles</i> mosquitoes collected between July 2021 and March 2023 from three Ethiopian sites (Lare, Asendabo, and Batu), representing differing insecticide use intensity and malaria transmission risk. Midgut microbiota of adult female <i>Anopheles funestus</i>, <i>An. coustani</i>, <i>An. pharaensis</i>, and both larvae and adult <i>Anopheles gambiae</i> s.l. were analysed using culture-dependent methods. Microbiota from insecticide-survived and susceptible <i>An. gambiae</i> s.l. were also examined under semi-field conditions. Generalized linear models (GLMs) with a negative binomial distribution and log link were used to assess the effects of mosquito species, site, and life stage on midgut bacterial count and composition. A total of 129 mosquito specimens were dissected, yielding 659 bacterial and 5 fungal isolates. Eleven bacterial genera were detected, dominated by <i>Staphylococcus</i> (32%), <i>Klebsiella</i> (17.5%), <i>Proteus</i> (11.4%), and <i>Bacillus</i> (11.7%). <i>Anopheles</i> species appear to be a key driver of midgut microbiota abundance (Wald χ² = 8.33, df = 3, <i>p</i> = 0.040), with insecticide survivors also showing numerically higher (63%, <i>n</i> = 113) culturable bacterial count and genus richness along with site-level differences as compared to bacterial counts recorded from the susceptible counterparts (37%, <i>n</i> = 66). <i>An. gambiae</i> s.l. harboured over 70% of bacterial counts, with larvae also showing high culturable colonization intensity. Bacterial load and diversity with Shannon index showed small differences and may not be biologically meaningful as no statistical comparison of diversity indices was performed. The composition and abundance of culturable midgut microbiota were influenced more by mosquito species than by environmental variation, with larvae and adults of <i>An. gambiae</i> s.l. harbouring relatively higher number of culturable fraction of bacterial isolates. This study provides baseline information on culturable midgut microbiota of <i>Anopheles</i> mosquitoes in malaria-endemic areas of Ethiopia and highlights the need for integrated culture-dependent and sequencing-based studies to better understand their functional roles and potential applications in malaria control.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Isolation and characterization of midgut microbiota in Anopheles mosquitoes from areas under varying insecticide pressure and malaria transmission settings of Ethiopia

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月20日

查看全文
  • Delelegn Woyessa,
  • Lijalem Hailu,
  • Delenasaw Yewhalaw

摘要

Investigating the composition and diversity of Anopheles midgut microbiota is essential for understanding vector–pathogen interactions, insecticide resistance, and malaria transmission dynamics across diverse eco-epidemiological settings. This study characterized culture-dependent midgut microbiota of Anopheles mosquitoes collected between July 2021 and March 2023 from three Ethiopian sites (Lare, Asendabo, and Batu), representing differing insecticide use intensity and malaria transmission risk. Midgut microbiota of adult female Anopheles funestus, An. coustani, An. pharaensis, and both larvae and adult Anopheles gambiae s.l. were analysed using culture-dependent methods. Microbiota from insecticide-survived and susceptible An. gambiae s.l. were also examined under semi-field conditions. Generalized linear models (GLMs) with a negative binomial distribution and log link were used to assess the effects of mosquito species, site, and life stage on midgut bacterial count and composition. A total of 129 mosquito specimens were dissected, yielding 659 bacterial and 5 fungal isolates. Eleven bacterial genera were detected, dominated by Staphylococcus (32%), Klebsiella (17.5%), Proteus (11.4%), and Bacillus (11.7%). Anopheles species appear to be a key driver of midgut microbiota abundance (Wald χ² = 8.33, df = 3, p = 0.040), with insecticide survivors also showing numerically higher (63%, n = 113) culturable bacterial count and genus richness along with site-level differences as compared to bacterial counts recorded from the susceptible counterparts (37%, n = 66). An. gambiae s.l. harboured over 70% of bacterial counts, with larvae also showing high culturable colonization intensity. Bacterial load and diversity with Shannon index showed small differences and may not be biologically meaningful as no statistical comparison of diversity indices was performed. The composition and abundance of culturable midgut microbiota were influenced more by mosquito species than by environmental variation, with larvae and adults of An. gambiae s.l. harbouring relatively higher number of culturable fraction of bacterial isolates. This study provides baseline information on culturable midgut microbiota of Anopheles mosquitoes in malaria-endemic areas of Ethiopia and highlights the need for integrated culture-dependent and sequencing-based studies to better understand their functional roles and potential applications in malaria control.