Scholarlink

<p>Strain MC2<sup>T</sup> was isolated from Hexachlorocyclohexane (HCH) contaminated soil, collected from a dumpsite at Ummari village, Uttar Pradesh, India. The isolate was characterized using a polyphasic taxonomic approach. Cells were Gram-positive, non-motile, short-rod, aerobic, catalase positive and oxidase negative capable of growth at 12–46 °C, pH 5–12 and up to 5% NaCl. Phylogenetic and phylogenomic analysis revealed that the strain is affiliated with the genus <i>Microbacterium</i>, sharing 98.64% 16S rRNA gene sequence similarity with <i>Microbacterium invictum</i> DC-200<sup>T</sup>. Overall genome related index (OGRI) analysis showed that strain MC2<sup>T</sup> and <i>Microbacterium invictum</i> DC-200<sup>T</sup> shares 26% digital DNA-DNA hybridization value and 83.02% Average Nucleotide Identity. Major polar lipids include phosphatidylglycerol, diphosphatidylglycerol, and one unknown lipid. The predominant respiratory quinones are MK-11 and MK-12. The cell-wall diamino acid is lysine, and anteiso-C₁₅:₀ is the major cellular fatty acid. Genome analysis unravelled the presence of biosynthetic gene clusters for carotenoids, ectoine and β-lactone. Based on a comprehensive comparison of the phenotypic, chemotaxonomic, phylogenetic and phylogenomic characteristics of closely related taxa, strain MC2<sup>T</sup> represents the novel species of the genus <i>Microbacterium</i>, for which the name <i>Microbacterium pollutisoli</i> sp. nov. (type strain MC2<sup>T</sup> =MCC 5196<sup>T</sup> =CCM 9353<sup>T</sup> = TSD 397<sup>T</sup>) is proposed.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Microbacterium pollutisoli sp. nov., isolated from HCH-contaminated soil, exhibits genomic potential for bioactive compounds

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月30日

查看全文
  • Monika Sharma,
  • Padma Dolkar,
  • Stanzin Choksket,
  • Suresh Korpole,
  • Ram Krishan Negi

摘要

Strain MC2T was isolated from Hexachlorocyclohexane (HCH) contaminated soil, collected from a dumpsite at Ummari village, Uttar Pradesh, India. The isolate was characterized using a polyphasic taxonomic approach. Cells were Gram-positive, non-motile, short-rod, aerobic, catalase positive and oxidase negative capable of growth at 12–46 °C, pH 5–12 and up to 5% NaCl. Phylogenetic and phylogenomic analysis revealed that the strain is affiliated with the genus Microbacterium, sharing 98.64% 16S rRNA gene sequence similarity with Microbacterium invictum DC-200T. Overall genome related index (OGRI) analysis showed that strain MC2T and Microbacterium invictum DC-200T shares 26% digital DNA-DNA hybridization value and 83.02% Average Nucleotide Identity. Major polar lipids include phosphatidylglycerol, diphosphatidylglycerol, and one unknown lipid. The predominant respiratory quinones are MK-11 and MK-12. The cell-wall diamino acid is lysine, and anteiso-C₁₅:₀ is the major cellular fatty acid. Genome analysis unravelled the presence of biosynthetic gene clusters for carotenoids, ectoine and β-lactone. Based on a comprehensive comparison of the phenotypic, chemotaxonomic, phylogenetic and phylogenomic characteristics of closely related taxa, strain MC2T represents the novel species of the genus Microbacterium, for which the name Microbacterium pollutisoli sp. nov. (type strain MC2T =MCC 5196T =CCM 9353T = TSD 397T) is proposed.