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<p>The finite proliferative lifespan and progressive loss of stemness in muscle stem cells present a significant bottleneck for the industrial-scale manufacturing of cultured meat. To overcome this limitation, we evaluated a microbial polysaccharide from <i>Metarhizium pinghaense</i> 15R (MP15R) for its capacity to augment bovine satellite cells (BSCs) functionality. Our in vitro findings demonstrate that MP15R treatment enhanced BSCs proliferation and migration while sustaining elevated expression of the canonical stem cell marker <i>PAX7</i> throughout prolonged passaging. Moreover, upon induction, MP15R robustly promoted terminal myogenic differentiation, evidenced by the upregulation of key markers such as <i>MYOG</i> and <i>MYHC</i> and an increased myotube fusion index. Collectively, these results establish the MP15R polysaccharide as a sustainable, cost-effective bioactive agent with a bifunctional capacity to improve both the expansion and differentiation phases essential for cultured meat bioprocessing.</p>

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Novel polysaccharides from Metarhizium pinghaense modulate bovine satellite cell fate for cultured meat production

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月22日

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  • J. H. Park,
  • J. W. Jang,
  • S. W. Jang,
  • Y. R. Kim,
  • J. H. Han,
  • G. R. Na,
  • Ikju. Park,
  • T. Y. Shin,
  • H. W. Choi

摘要

The finite proliferative lifespan and progressive loss of stemness in muscle stem cells present a significant bottleneck for the industrial-scale manufacturing of cultured meat. To overcome this limitation, we evaluated a microbial polysaccharide from Metarhizium pinghaense 15R (MP15R) for its capacity to augment bovine satellite cells (BSCs) functionality. Our in vitro findings demonstrate that MP15R treatment enhanced BSCs proliferation and migration while sustaining elevated expression of the canonical stem cell marker PAX7 throughout prolonged passaging. Moreover, upon induction, MP15R robustly promoted terminal myogenic differentiation, evidenced by the upregulation of key markers such as MYOG and MYHC and an increased myotube fusion index. Collectively, these results establish the MP15R polysaccharide as a sustainable, cost-effective bioactive agent with a bifunctional capacity to improve both the expansion and differentiation phases essential for cultured meat bioprocessing.