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<p>This study aimed to investigate the effects of extracellular adenosine triphosphate (eATP) on the migration and extracellular matrix (ECM) homeostasis of oral squamous cell carcinoma (OSCC) cells. Two tongue-origin OSCC cell lines, HSC-4 (metastatic) and HSC-7 (primary), were treated with various concentrations of eATP (0–200 µM). Cell viability and migration were assessed using MTT and scratch assays. The mRNA expression of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) was analyzed by RT-qPCR. MMP9 and MMP3 release was detected by ELISA. P<sub>2</sub>Y<sub>1</sub> and P<sub>2</sub>Y<sub>2</sub> involvement was assessed using MRS2179 and AR-C118925, respectively. Both cell lines expressed numerous P<sub>2</sub> receptors. 50 µM eATP selectively downregulated <i>MMP8</i> and <i>MMP9</i> expression in both lines and reduced MMP9 and MMP3 secretion, whereas <i>TIMP</i> levels remained unchanged. P<sub>2</sub>Y<sub>1</sub> inhibition reversed eATP-mediated MMP suppression, whereas P<sub>2</sub>Y<sub>2</sub> inhibition suppressed MMP expression. eATP influenced cell proliferation in both cell lines at different time points. 200 µM eATP reduced migration and increased E-cadherin expression in HSC-4, whereas no migratory changes were observed in HSC-7. eATP modulates OSCC migration and ECM homeostasis. These findings indicate that eATP-purinergic signaling plays a pivotal role in ECM dynamics in OSCC and is a potential target for effective treatment of tongue-origin OSCC.</p>

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Adenosine triphosphate regulates metastatic behaviors of oral squamous cell carcinoma cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月20日

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  • Phawilai Prachathai,
  • Sanicha Yaklai,
  • Maythwe Kyawsoewin,
  • Pawat Sripodok,
  • Watcharaphol Tiskratok,
  • Hiroshi Egusa,
  • Thanaphum Osathanon,
  • Phoonsuk Limraksasin

摘要

This study aimed to investigate the effects of extracellular adenosine triphosphate (eATP) on the migration and extracellular matrix (ECM) homeostasis of oral squamous cell carcinoma (OSCC) cells. Two tongue-origin OSCC cell lines, HSC-4 (metastatic) and HSC-7 (primary), were treated with various concentrations of eATP (0–200 µM). Cell viability and migration were assessed using MTT and scratch assays. The mRNA expression of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) was analyzed by RT-qPCR. MMP9 and MMP3 release was detected by ELISA. P2Y1 and P2Y2 involvement was assessed using MRS2179 and AR-C118925, respectively. Both cell lines expressed numerous P2 receptors. 50 µM eATP selectively downregulated MMP8 and MMP9 expression in both lines and reduced MMP9 and MMP3 secretion, whereas TIMP levels remained unchanged. P2Y1 inhibition reversed eATP-mediated MMP suppression, whereas P2Y2 inhibition suppressed MMP expression. eATP influenced cell proliferation in both cell lines at different time points. 200 µM eATP reduced migration and increased E-cadherin expression in HSC-4, whereas no migratory changes were observed in HSC-7. eATP modulates OSCC migration and ECM homeostasis. These findings indicate that eATP-purinergic signaling plays a pivotal role in ECM dynamics in OSCC and is a potential target for effective treatment of tongue-origin OSCC.