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<p>Newcastle disease virus is one of the most highly contagious infections impacting poultry industries worldwide. This study aimed to develop a bacteriophage display system as a cost-effective and immunogenic method for expressing the immunodominant region of the Newcastle Disease Virus Fusion (F) protein for use in serological assays. Both <i>in-silico</i> and <i>in-vitro</i> analyses indicated that the selected antigen is stable, accurate, and reliable for further <i>in-vivo</i> studies. The displayed antigen successfully detected NDV-GVII-specific antibodies in vaccinated chickens. Additionally, a significant correlation was observed between ELISA and HI test results on chicken serum samples. This approach addresses safety concerns associated with wild-type virulent NDVs and offers improved diagnostic accuracy and scalability, aligning with industry needs for improved Newcastle disease detection, control, and management on poultry farms.</p>

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Development of a novel phage-displayed fusion protein of newcastle disease virus as a highly specific antigen for serological assays

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月20日

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  • Mehrnaz Najafi,
  • Hanh Thi Hong Nguyen,
  • Bahareh Pakbaten,
  • Aliakbar Khabiri,
  • Abdollah Derakhshandeh,
  • Chen Ling,
  • Ni Luh Puti Indi Dharmayanti,
  • Harimurti Nuradji,
  • Faidah Rahmawati,
  • Rahmat Setya Adji,
  • Nuha Fairunisya,
  • Fitirine Ekawasti,
  • Prima Mei Widiy,
  • Tati Ariyanti,
  • Ngoc X. B. Nguyen,
  • Kandarp Patel,
  • Maryam Sadat Moezzi,
  • Mohammad Indro Cahyono,
  • Sammi Yuen Tung Ngai,
  • Putri Pandarangga,
  • Farhid Hemmatzadeh

摘要

Newcastle disease virus is one of the most highly contagious infections impacting poultry industries worldwide. This study aimed to develop a bacteriophage display system as a cost-effective and immunogenic method for expressing the immunodominant region of the Newcastle Disease Virus Fusion (F) protein for use in serological assays. Both in-silico and in-vitro analyses indicated that the selected antigen is stable, accurate, and reliable for further in-vivo studies. The displayed antigen successfully detected NDV-GVII-specific antibodies in vaccinated chickens. Additionally, a significant correlation was observed between ELISA and HI test results on chicken serum samples. This approach addresses safety concerns associated with wild-type virulent NDVs and offers improved diagnostic accuracy and scalability, aligning with industry needs for improved Newcastle disease detection, control, and management on poultry farms.