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<p>This study aims to assess the impact of pre-clinical (ex vivo) use including autoclave sterilization on the surface roughness of reciprocating NiTi instruments using a new treatment of two images with different angles captured by field-emission scanning electron microscopy (FE-SEM) and Mountains software. A pre-clinical study was conducted to evaluate surface roughness changes in NiTi instruments after three instrumentations. Using a repeated-measures design in which each instrument served as its own control, Instruments were examined using FE-SEM and analyzed with Mountains software to quantify roughness parameters (Sa, Sq, Sz) at two locations: the cutting edge and the adjacent flute. Three-dimensional surfaces were reconstructed from dual-angle stereo-pair micrographs per ISO 25178-2. Pre- and post-instrumentation roughness measurements were statistically compared using repeated-measures ANOVA and Friedman tests. There was no significant difference in surface roughness between experimental groups in the pre-clinical environment at the edge of the One RECI instrument. However, there is a linear trend of the increase of the Sa and Sq parameters at the cutting edge of the instrument after several human-extracted root canal instrumentations. There is a significant difference in surface roughness in Sa parameter between experimental groups in the pre-clinical environment at the flute of the One RECI instrument. Pre-clinical use leads to measurable increases in surface roughness in reciprocating NiTi instruments, particularly at the cutting-edge. These findings highlight the importance of monitoring instrument wear to ensure optimal performance and safety.</p>

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A novel quantitative method for evaluating surface roughness using dual-angle images from a field emission scanning electron microscope

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月21日

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  • Nguyen-Huu-Phuoc Huynh,
  • Hong-Hai Le,
  • Hoang-Vinh Le,
  • Thu-Tra Nguyen,
  • Huynh-Anh Bui,
  • Thi-Thuy-Trang Huynh,
  • Hoang-Lan-Anh Le,
  • Phuong-Doan Phan,
  • Nguyen-Tra-Mi Le,
  • Thuan-Loc Tran,
  • Trieu-Khang Pham,
  • Cong-Minh Nguyen,
  • Huynh-Thuy-Anh Bui,
  • Thi-Bich-Van Tran,
  • Quoc-Viet Lam,
  • Dai-Phong Lam,
  • Thi-Nguyet-Anh Nguyen,
  • Van-Khoa Pham,
  • An-Tran Pham,
  • Tran-Lan-Khue Pham

摘要

This study aims to assess the impact of pre-clinical (ex vivo) use including autoclave sterilization on the surface roughness of reciprocating NiTi instruments using a new treatment of two images with different angles captured by field-emission scanning electron microscopy (FE-SEM) and Mountains software. A pre-clinical study was conducted to evaluate surface roughness changes in NiTi instruments after three instrumentations. Using a repeated-measures design in which each instrument served as its own control, Instruments were examined using FE-SEM and analyzed with Mountains software to quantify roughness parameters (Sa, Sq, Sz) at two locations: the cutting edge and the adjacent flute. Three-dimensional surfaces were reconstructed from dual-angle stereo-pair micrographs per ISO 25178-2. Pre- and post-instrumentation roughness measurements were statistically compared using repeated-measures ANOVA and Friedman tests. There was no significant difference in surface roughness between experimental groups in the pre-clinical environment at the edge of the One RECI instrument. However, there is a linear trend of the increase of the Sa and Sq parameters at the cutting edge of the instrument after several human-extracted root canal instrumentations. There is a significant difference in surface roughness in Sa parameter between experimental groups in the pre-clinical environment at the flute of the One RECI instrument. Pre-clinical use leads to measurable increases in surface roughness in reciprocating NiTi instruments, particularly at the cutting-edge. These findings highlight the importance of monitoring instrument wear to ensure optimal performance and safety.