Scholarlink

<p>Cutaneous squamous cell carcinoma (cSCC) arises from epidermal keratinocytes and is the second most common epithelial malignancy. E74-like factor 5 (ELF5) is a member of the epithelium specific ETS subfamily of transcription factors and is essential for epithelial development, homeostasis and prevention of epithelial tumourigenesis. Transformation of keratinocytes involves the disruption of the progenitor differentiation program causing keratinocytes to fail to complete the process. This can lead to dysplastic epithelium and precancerous cells in skin. However, the precise mechanisms that causes the failure of keratinocytes to differentiate completely is not well understood. In our study, we discovered that ELF5 is abundantly expressed in healthy human skin (both basal and suprabasal layers), while its expression is lost in cSCC tissue biopsies. Using CRISPR-Cas9 knockout technology, functional studies along with transcript and protein analysis, loss of ELF5 in immortalised healthy keratinocytes leads to decreased ability of cells to differentiation and increased migration and tumourigenicity, in vitro. Overexpression of ELF5 leads to inhibition of cell migration and proliferation in keratinocytes and in cancer cell lines (A431 and SCC-9), in vitro. Further analysis using RNA transcriptomics has uncovered that GATA-3 and FLI-1 are potentially key molecular targets of ELF5 in keratinocytes. ELF5 regulation of both GATA-3 and FLI-1 is required to maintain the balance between healthy keratinocytes (proliferation/differentiation) and to inhibit precancerous cell formation (tumourigenicity). Our data indicates that ELF5 plays an important role in potentially preventing transformation of keratinocytes and could suppresses skin tumourigenesis. However, additional studies are required to determine this, which could lead the targeting of ELF5 for future drug treatments in cSCC. Better understanding of the underlying mechanisms by which ELF5 regulates gene expression and consequently cell activity will provide new knowledge that can be translated into long-term benefits for patients with precancerous lesions to cSCC.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Interplay of ELF5, GATA-3 and FLI-1 regulate malignant transformation of keratinocytes

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月16日

查看全文
  • Hetal. J. Patel,
  • Maximilian. E. Pickup,
  • Maryam. A. Lawal,
  • Chandrakala Khadka,
  • Mohammed. I. Ahmed

摘要

Cutaneous squamous cell carcinoma (cSCC) arises from epidermal keratinocytes and is the second most common epithelial malignancy. E74-like factor 5 (ELF5) is a member of the epithelium specific ETS subfamily of transcription factors and is essential for epithelial development, homeostasis and prevention of epithelial tumourigenesis. Transformation of keratinocytes involves the disruption of the progenitor differentiation program causing keratinocytes to fail to complete the process. This can lead to dysplastic epithelium and precancerous cells in skin. However, the precise mechanisms that causes the failure of keratinocytes to differentiate completely is not well understood. In our study, we discovered that ELF5 is abundantly expressed in healthy human skin (both basal and suprabasal layers), while its expression is lost in cSCC tissue biopsies. Using CRISPR-Cas9 knockout technology, functional studies along with transcript and protein analysis, loss of ELF5 in immortalised healthy keratinocytes leads to decreased ability of cells to differentiation and increased migration and tumourigenicity, in vitro. Overexpression of ELF5 leads to inhibition of cell migration and proliferation in keratinocytes and in cancer cell lines (A431 and SCC-9), in vitro. Further analysis using RNA transcriptomics has uncovered that GATA-3 and FLI-1 are potentially key molecular targets of ELF5 in keratinocytes. ELF5 regulation of both GATA-3 and FLI-1 is required to maintain the balance between healthy keratinocytes (proliferation/differentiation) and to inhibit precancerous cell formation (tumourigenicity). Our data indicates that ELF5 plays an important role in potentially preventing transformation of keratinocytes and could suppresses skin tumourigenesis. However, additional studies are required to determine this, which could lead the targeting of ELF5 for future drug treatments in cSCC. Better understanding of the underlying mechanisms by which ELF5 regulates gene expression and consequently cell activity will provide new knowledge that can be translated into long-term benefits for patients with precancerous lesions to cSCC.