<p>Although alfalfa is capable of nitrogen fixation, the optimal nitrogen (N) application rate for jujube-alfalfa intercropping systems in arid oasis regions remains unclear. This study evaluated four N application rates (0, 80, 160, and 240&#xa0;kg/ha) to determine their effects on alfalfa photosynthesis, yield, and quality. Measurements of gas exchange and chlorophyll fluorescence revealed that the 160&#xa0;kg/ha treatment (N2) significantly enhanced photosynthetic performance, particularly at the early flowering stage—a critical period when peak photosynthetic activity directly governs biomass accumulation and final yield. At this stage, the net photosynthetic rate (Pn) in the first and second harvests increased by 31.1% and 42.8%, respectively, while the photosynthetic performance index (PIabs) improved by 37.7%–102.2%. Compared with the unfertilized control, the N2 treatment significantly increased fresh forage yield by 23.8%–45.8% and dry matter yield by 18.4%–29.7%; crude protein content rose by up to 9.6% in the second harvest. Yield was significantly positively correlated with Pn, transpiration rate (Tr), the maximum photochemical efficiency of PSII (Fv/Fm), and PIabs. In conclusion, a nitrogen application rate of 160&#xa0;kg/ha is recommended to maximize alfalfa yield and quality in this intercropping system.</p>

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Optimizing nitrogen management enhances photosynthesis and alfalfa productivity in jujube-alfalfa intercropping

  • Gulinigaer Taxi,
  • Ling Li,
  • Jinbin Wang,
  • Zhengjun Cui,
  • Tiantian Li,
  • Yaru Zhao,
  • Hang Qiao,
  • Sumei Wan,
  • Xiao-Fei Li,
  • Wei Zhang,
  • Hong Li,
  • Guodong Chen

摘要

Although alfalfa is capable of nitrogen fixation, the optimal nitrogen (N) application rate for jujube-alfalfa intercropping systems in arid oasis regions remains unclear. This study evaluated four N application rates (0, 80, 160, and 240 kg/ha) to determine their effects on alfalfa photosynthesis, yield, and quality. Measurements of gas exchange and chlorophyll fluorescence revealed that the 160 kg/ha treatment (N2) significantly enhanced photosynthetic performance, particularly at the early flowering stage—a critical period when peak photosynthetic activity directly governs biomass accumulation and final yield. At this stage, the net photosynthetic rate (Pn) in the first and second harvests increased by 31.1% and 42.8%, respectively, while the photosynthetic performance index (PIabs) improved by 37.7%–102.2%. Compared with the unfertilized control, the N2 treatment significantly increased fresh forage yield by 23.8%–45.8% and dry matter yield by 18.4%–29.7%; crude protein content rose by up to 9.6% in the second harvest. Yield was significantly positively correlated with Pn, transpiration rate (Tr), the maximum photochemical efficiency of PSII (Fv/Fm), and PIabs. In conclusion, a nitrogen application rate of 160 kg/ha is recommended to maximize alfalfa yield and quality in this intercropping system.