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<p>The presented study is focused on evaluating the efficiency of repurposing Febuxostat (FBX) as a chemotherapeutic newcomer against the cervical cancer cell line (Hela cells). Exploiting nanotechnology benefits in drug delivery, FBX was incorporated into chitosan-coated niosomes (Chitosomes). Using a 2<sup>3</sup> factorial design, eight formulations were characterized for their entrapment efficiency percentage, particle size, and zeta potential. The optimum (FBX) loaded chitosomes displayed a mean particle size of 339 ± 14&#xa0;nm, an entrapment efficiency of 91.07 ± 0.33%, and a Zeta potential of + 26.9 ± 1.2 ± 1.2 mV. In vitro cytotoxicity studies performed on Hela cells indicated a significant (<i>P</i> &lt; 0.05) decrease in IC<sub>50</sub> value around 3-fold compared with pure FBX. The cellular uptake showed a 2-fold increase compared to the free drug. Upon studying the cell death cycle, it was revealed that apoptotic cell death was caused by the drug in the G1/S phase. Altogether, these findings revealed that the optimized chitosomal dispersion exhibited superior cytotoxic activity compared to free FBX, indicating it as a promising efficient and biocompatible delivery system for cervical cancer treatment.</p>

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Formulation, characterization and evaluation of cytotoxic potential for febuxostat loaded chitosomes against cervical cancer cells (Hela cells)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月21日

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  • Wedad Sakran,
  • Mai Abdel‑Hakim,
  • Rania S. Abdel‑Rashid

摘要

The presented study is focused on evaluating the efficiency of repurposing Febuxostat (FBX) as a chemotherapeutic newcomer against the cervical cancer cell line (Hela cells). Exploiting nanotechnology benefits in drug delivery, FBX was incorporated into chitosan-coated niosomes (Chitosomes). Using a 23 factorial design, eight formulations were characterized for their entrapment efficiency percentage, particle size, and zeta potential. The optimum (FBX) loaded chitosomes displayed a mean particle size of 339 ± 14 nm, an entrapment efficiency of 91.07 ± 0.33%, and a Zeta potential of + 26.9 ± 1.2 ± 1.2 mV. In vitro cytotoxicity studies performed on Hela cells indicated a significant (P < 0.05) decrease in IC50 value around 3-fold compared with pure FBX. The cellular uptake showed a 2-fold increase compared to the free drug. Upon studying the cell death cycle, it was revealed that apoptotic cell death was caused by the drug in the G1/S phase. Altogether, these findings revealed that the optimized chitosomal dispersion exhibited superior cytotoxic activity compared to free FBX, indicating it as a promising efficient and biocompatible delivery system for cervical cancer treatment.