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<p>Circular RNAs (circRNAs) are a type of non-coding RNA molecule whose functional processes in carcinogenesis and progression are not fully known. Recent studies suggest that circRNAs can act as functional RNAs involved in the progression of various tumors. This study aims to investigate the role of hsa_circ_0085465 in the proliferation, migration, and invasion processes of gastric cancer cells. By establishing models of <i>Helicobacter pylori</i> (<i>H. pylori</i>)-infected normal gastric epithelial cells and gastric cancer cells, we conducted whole transcriptome sequencing and bioinformatics analysis, which led to the identification of a significantly upregulated circular RNA molecule, hsa_circ_0085465. Subsequently, we investigated the function of hsa_circ_0085465 in gastric cancer through in vitro functional experiments, including scratch wound healing assays and transwell assays, as well as in vivo animal experiments. Then, the downstream target miRNA of hsa_circ_0085465 were identified using sequencing and bioinformatics analysis, and the binding between hsa_circ_0085465 and the target gene miR-33b-3p was validated using dual-luciferase reporter gene assays and fluorescence in situ hybridization (FISH). Finally, we explored the interaction between hsa_circ_0085465 and miR-33b-3p through in vitro functional analyses. In a gastric cell model infected with <i>H. pylori</i>, the expression level of hsa_circ_0085465 was significantly upregulated. In vitro experiments demonstrated that hsa_circ_0085465 promotes the proliferation, migration, and invasion of gastric cancer cells, while in vivo subcutaneous tumor model further confirmed that hsa_circ_0085465 markedly accelerates tumor growth. Subsequent analyses identified miR-33b-3p as a downstream target of hsa_circ_0085465, and miR-33b-3p was found to suppress the proliferation, migration, and invasion of gastric cancer cells. Rescue experiments further demonstrated that miR-33b-3p can partially reverse the oncogenic effects of hsa_circ_0085465 in gastric cancer. <i>H. pylori</i> induced the upregulation of hsa_circ_0085465 in gastric cells, and hsa_circ_0085465 functions as a molecular sponge for miR-33b-3p, thereby promoting the proliferation, migration, and invasion of gastric cancer cells.</p>

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H. pylori-induced hsa_circ_0085465 promotes gastric cancer cell proliferation, migration, and invasion by sponging miR-33b-3p

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月26日

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  • Jing Wu,
  • Hongpeng Liu,
  • Furui Zhang,
  • Jiale Chen,
  • Kunmei Liu,
  • Juan Chen,
  • Le Guo,
  • Zhen Zhang

摘要

Circular RNAs (circRNAs) are a type of non-coding RNA molecule whose functional processes in carcinogenesis and progression are not fully known. Recent studies suggest that circRNAs can act as functional RNAs involved in the progression of various tumors. This study aims to investigate the role of hsa_circ_0085465 in the proliferation, migration, and invasion processes of gastric cancer cells. By establishing models of Helicobacter pylori (H. pylori)-infected normal gastric epithelial cells and gastric cancer cells, we conducted whole transcriptome sequencing and bioinformatics analysis, which led to the identification of a significantly upregulated circular RNA molecule, hsa_circ_0085465. Subsequently, we investigated the function of hsa_circ_0085465 in gastric cancer through in vitro functional experiments, including scratch wound healing assays and transwell assays, as well as in vivo animal experiments. Then, the downstream target miRNA of hsa_circ_0085465 were identified using sequencing and bioinformatics analysis, and the binding between hsa_circ_0085465 and the target gene miR-33b-3p was validated using dual-luciferase reporter gene assays and fluorescence in situ hybridization (FISH). Finally, we explored the interaction between hsa_circ_0085465 and miR-33b-3p through in vitro functional analyses. In a gastric cell model infected with H. pylori, the expression level of hsa_circ_0085465 was significantly upregulated. In vitro experiments demonstrated that hsa_circ_0085465 promotes the proliferation, migration, and invasion of gastric cancer cells, while in vivo subcutaneous tumor model further confirmed that hsa_circ_0085465 markedly accelerates tumor growth. Subsequent analyses identified miR-33b-3p as a downstream target of hsa_circ_0085465, and miR-33b-3p was found to suppress the proliferation, migration, and invasion of gastric cancer cells. Rescue experiments further demonstrated that miR-33b-3p can partially reverse the oncogenic effects of hsa_circ_0085465 in gastric cancer. H. pylori induced the upregulation of hsa_circ_0085465 in gastric cells, and hsa_circ_0085465 functions as a molecular sponge for miR-33b-3p, thereby promoting the proliferation, migration, and invasion of gastric cancer cells.