Scholarlink

<p>As there is a lack of reliable structural outcome parameters for posterior uveitis trials, we compared inter-rater reliability (IRR) of chorioretinal lesion area measurement (quantitative analyses) and characterization (qualitative analyses) on color fundus photography (CFP) and multimodal fundus autofluorescence (FAF). In this prospective cohort study, posterior uveitis eyes were imaged with CFP (Eidon, iCare, Vantaa), short- (swBAF, 450&#xa0;nm; Eidon) and long-wavelength blue-light-autofluorescence (lwBAF, 488&#xa0;nm), green-light-autofluorescence (GAF, 518&#xa0;nm), and infrared-autofluorescence (IRAF, 787&#xa0;nm) (all Spectralis, Heidelberg Engineering, Heidelberg). Lesion area, measured with ImageJ (National Institutes of Health), and image characteristics of lesions were graded on all modalities by two masked raters. A total of 318 lesions from 27 eyes (17 patients) were included. Absolute inter-rater differences in area measurement were 0.57, 0.78, 0.30, 0.36, and 0.55 in standardized 10<sup>3</sup> pixels for CFP, swBAF, lwBAF, GAF, and IRAF, respectively. IRR was high for all modalities for quantitative (intraclass correlation, 95% confidence interval (CI) [0.997–0.998]) and at least substantial for qualitative measures (unweighted Cohen’s kappa 0.84, 0.91, 0.89, 0.91, and 0.89 for CFP, swBAF, lwBAF, GAF, and IRAF, respectively, all <i>p</i> &lt; 0.0001, CIs [0.79–0.95]). Hence, FAF could be a reliable complementary imaging modality for posterior uveitis clinical trials, especially for lesion quantification.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Reliability of quantitative and qualitative chorioretinal uveitis lesion analysis on blue, green, and near-infrared fundus autofluorescence and color fundus photography

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月1日

查看全文
  • Marie D. Just,
  • Jana Koch,
  • Gabriela Guzman,
  • Lennart J. Overbeck,
  • Moritz Berger,
  • Jan H. Terheyden,
  • Selina Foti,
  • Matthias Schmid,
  • Frank G. Holz,
  • Marlene Saßmannshausen,
  • Matthias M. Mauschitz,
  • Robert P. Finger,
  • Maximilian W. M. Wintergerst

摘要

As there is a lack of reliable structural outcome parameters for posterior uveitis trials, we compared inter-rater reliability (IRR) of chorioretinal lesion area measurement (quantitative analyses) and characterization (qualitative analyses) on color fundus photography (CFP) and multimodal fundus autofluorescence (FAF). In this prospective cohort study, posterior uveitis eyes were imaged with CFP (Eidon, iCare, Vantaa), short- (swBAF, 450 nm; Eidon) and long-wavelength blue-light-autofluorescence (lwBAF, 488 nm), green-light-autofluorescence (GAF, 518 nm), and infrared-autofluorescence (IRAF, 787 nm) (all Spectralis, Heidelberg Engineering, Heidelberg). Lesion area, measured with ImageJ (National Institutes of Health), and image characteristics of lesions were graded on all modalities by two masked raters. A total of 318 lesions from 27 eyes (17 patients) were included. Absolute inter-rater differences in area measurement were 0.57, 0.78, 0.30, 0.36, and 0.55 in standardized 103 pixels for CFP, swBAF, lwBAF, GAF, and IRAF, respectively. IRR was high for all modalities for quantitative (intraclass correlation, 95% confidence interval (CI) [0.997–0.998]) and at least substantial for qualitative measures (unweighted Cohen’s kappa 0.84, 0.91, 0.89, 0.91, and 0.89 for CFP, swBAF, lwBAF, GAF, and IRAF, respectively, all p < 0.0001, CIs [0.79–0.95]). Hence, FAF could be a reliable complementary imaging modality for posterior uveitis clinical trials, especially for lesion quantification.