Scholarlink

<p>The exploration of extremotolerant microorganisms from arid ecosystems offers promising avenues for novel bioactive compounds discovery. In this study, a radioresistant actinobacterial strain, CH-8, was isolated from the hyper-arid soil of the Cholistan Desert, Pakistan. Phylogenetic analysis of the 16&#xa0;S rRNA gene revealed 99.88% similarity with <i>Streptomyces alkaliterrae</i>. Strain CH-8 demonstrated high oxidative and UV-B tolerance, with 78% and 60% survival under 10 mM H<sub>2</sub>O<sub>2</sub> and 2712&#xa0;J/m<sup>2</sup> UV-B exposure, respectively. Intracellular metabolites were extracted using methanol and purified via silica gel chromatography. The purified fraction showed strong antioxidant activity (78% DPPH scavenging), and was rich in phenolics (149&#xa0;mg GAE/g) and flavonoids (2.082&#xa0;mg QE/g). Crude extracts exhibited significant anti-biofilm activity against Gram-positive pathogens, achieving up to 85.4% inhibition and 88.1% eradication. Cytotoxic potential was confirmed via brine shrimp lethality assay (IC<sub>50</sub> = 19.23&#xa0;µg/mL), and DNA damage protection indicated radioprotective capability. LC-MS analysis identified several bioactive compounds, including a glycosylated polyketide (m/z 575.6) resembling dynemicin L, as well as lankacidin C and lynamicin D. These findings position <i>S. alkaliterrae</i> CH-8 as a promising source of therapeutic secondary metabolites, and highlight the Cholistan Desert as a reservoir of untapped microbial and biochemical diversity.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Bioactive metabolites of UV-resistant Streptomyces alkaliterrae CH-8 and their antioxidant and antibiofilm potential

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月20日

查看全文
  • Tayyaba Alam,
  • Salah Ud Din,
  • Afaq Ahmad,
  • Mahwish Ali,
  • Malik Badshah,
  • Samiullah Khan,
  • Fariha Hasan,
  • Muhammad Farman,
  • Aamer Ali Shah

摘要

The exploration of extremotolerant microorganisms from arid ecosystems offers promising avenues for novel bioactive compounds discovery. In this study, a radioresistant actinobacterial strain, CH-8, was isolated from the hyper-arid soil of the Cholistan Desert, Pakistan. Phylogenetic analysis of the 16 S rRNA gene revealed 99.88% similarity with Streptomyces alkaliterrae. Strain CH-8 demonstrated high oxidative and UV-B tolerance, with 78% and 60% survival under 10 mM H2O2 and 2712 J/m2 UV-B exposure, respectively. Intracellular metabolites were extracted using methanol and purified via silica gel chromatography. The purified fraction showed strong antioxidant activity (78% DPPH scavenging), and was rich in phenolics (149 mg GAE/g) and flavonoids (2.082 mg QE/g). Crude extracts exhibited significant anti-biofilm activity against Gram-positive pathogens, achieving up to 85.4% inhibition and 88.1% eradication. Cytotoxic potential was confirmed via brine shrimp lethality assay (IC50 = 19.23 µg/mL), and DNA damage protection indicated radioprotective capability. LC-MS analysis identified several bioactive compounds, including a glycosylated polyketide (m/z 575.6) resembling dynemicin L, as well as lankacidin C and lynamicin D. These findings position S. alkaliterrae CH-8 as a promising source of therapeutic secondary metabolites, and highlight the Cholistan Desert as a reservoir of untapped microbial and biochemical diversity.