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<p>Post-hepatectomy liver failure is a serious complication following liver resection and is associated with high morbidity and mortality. Platelets play a crucial role in liver regeneration due to their rich content of growth factors. However, the comparative effects of platelet-rich plasma (PRP) and concentrated growth factor (CGF) on liver regeneration remain unclear. This study aimed to evaluate and compare the effects of splenectomy (SPx), PRP, and CGF on liver regeneration and function following 70% hepatectomy in rats. Male Wistar rats were randomly divided into six groups: Control, Sham, Hepatectomy (PHx), Hepatectomy + SPx, Hepatectomy + PRP, and PHx + CGF. PRP and CGF were prepared using differential centrifugation and administered via the portal vein after hepatectomy. Liver regeneration was assessed histopathologically, immunohistochemically and biochemically on postoperative day (POD) 5. PRP treatment significantly increased Ki-67 expression, indicating enhanced hepatocyte proliferation. CGF promoted neovascularization and improved hepatocyte morphology but showed lower Ki-67 expression than PRP. SPx did not significantly enhance regeneration compared to PHx alone. Signal Transducer and Activator of Transcription 3 (STAT3) expression was highest in the PHx group, whereas the PRP and CGF groups exhibited moderate levels. PRP and CGF improved liver function biochemically, reducing bilirubin levels. Both PRP and CGF promote liver regeneration, albeit through distinct mechanisms: PRP primarily enhances cellular proliferation, whereas CGF facilitates vascularization and modulates the immune response. SPx did not confer additional benefits in this model. These findings suggest that PRP and CGF may have potential therapeutic applications in preventing post-hepatectomy liver failure.</p>

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The effects of splenectomy, platelet-rich plasma (PRP) and concentrated growth factor (CGF) on liver regeneration following major hepatectomy in rats

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月25日

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  • Yunus Emre Sacin,
  • Esma Kirimlioglu,
  • Ulas Ural,
  • Asuman Kaplan Algın,
  • Ali Avanaz,
  • Mustafa Ormeci,
  • Guliz Avsar,
  • Abdullah Kısaoglu

摘要

Post-hepatectomy liver failure is a serious complication following liver resection and is associated with high morbidity and mortality. Platelets play a crucial role in liver regeneration due to their rich content of growth factors. However, the comparative effects of platelet-rich plasma (PRP) and concentrated growth factor (CGF) on liver regeneration remain unclear. This study aimed to evaluate and compare the effects of splenectomy (SPx), PRP, and CGF on liver regeneration and function following 70% hepatectomy in rats. Male Wistar rats were randomly divided into six groups: Control, Sham, Hepatectomy (PHx), Hepatectomy + SPx, Hepatectomy + PRP, and PHx + CGF. PRP and CGF were prepared using differential centrifugation and administered via the portal vein after hepatectomy. Liver regeneration was assessed histopathologically, immunohistochemically and biochemically on postoperative day (POD) 5. PRP treatment significantly increased Ki-67 expression, indicating enhanced hepatocyte proliferation. CGF promoted neovascularization and improved hepatocyte morphology but showed lower Ki-67 expression than PRP. SPx did not significantly enhance regeneration compared to PHx alone. Signal Transducer and Activator of Transcription 3 (STAT3) expression was highest in the PHx group, whereas the PRP and CGF groups exhibited moderate levels. PRP and CGF improved liver function biochemically, reducing bilirubin levels. Both PRP and CGF promote liver regeneration, albeit through distinct mechanisms: PRP primarily enhances cellular proliferation, whereas CGF facilitates vascularization and modulates the immune response. SPx did not confer additional benefits in this model. These findings suggest that PRP and CGF may have potential therapeutic applications in preventing post-hepatectomy liver failure.