Scholarlink

<p>The endophytic mycoflora of the bark tissue of <i>Sorbus commixta</i> sampled from Hokkaido, Japan was investigated. Bark pieces were surface-sterilized and placed on potato dextrose agar to extract template DNA from the fungi. Twenty-one operational taxonomic units (OTUs) were isolated from whole and outer bark segments, with colonization frequencies of 20% and 43%, respectively. The fungal composition included 20% OTUs of <i>Pezicula sporulosa</i> and <i>Xylaria</i> spp. in the whole bark, 43% OTUs of <i>Phaemoniella</i> spp., and 24% OTUs of <i>Helminthosporium</i> spp. in the outer bark. Endophytic fungi were not detected in the inner bark segments on the agar. In addition, we examined the antifungal activity (AFA) of <i>S. commixta</i> inner bark against <i>Trametes versicolor</i> and endophytic fungi, including <i>P. sporulosa</i> and <i>Diaporthe</i> spp. We found that the inner bark exhibited selective AFA against endophytic fungi; while inhibition was significant for some, it was lower than the complete inhibition observed against <i>T. versicolor</i>. In conclusion, <i>S. commixta</i> provides a habitat for endophytic fungi in the outer bark, but little or no habitat in the inner bark, likely due to the selective pressure of hydrogen cyanide from cyanogenic glycosides in the inner bark.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Differential inhibition of bark endophytes and a wood-decay fungus by inner bark of Sorbus commixta

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月10日

查看全文
  • Masato Koyama,
  • Yutaka Tamai,
  • Kengo Shigetomi,
  • Yuzou Sano

摘要

The endophytic mycoflora of the bark tissue of Sorbus commixta sampled from Hokkaido, Japan was investigated. Bark pieces were surface-sterilized and placed on potato dextrose agar to extract template DNA from the fungi. Twenty-one operational taxonomic units (OTUs) were isolated from whole and outer bark segments, with colonization frequencies of 20% and 43%, respectively. The fungal composition included 20% OTUs of Pezicula sporulosa and Xylaria spp. in the whole bark, 43% OTUs of Phaemoniella spp., and 24% OTUs of Helminthosporium spp. in the outer bark. Endophytic fungi were not detected in the inner bark segments on the agar. In addition, we examined the antifungal activity (AFA) of S. commixta inner bark against Trametes versicolor and endophytic fungi, including P. sporulosa and Diaporthe spp. We found that the inner bark exhibited selective AFA against endophytic fungi; while inhibition was significant for some, it was lower than the complete inhibition observed against T. versicolor. In conclusion, S. commixta provides a habitat for endophytic fungi in the outer bark, but little or no habitat in the inner bark, likely due to the selective pressure of hydrogen cyanide from cyanogenic glycosides in the inner bark.