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<p>The present study comprehensively investigated the probiotic and anti-biofilm potential of the <i>Lactobacillus acidophilus</i> TMPC 41127 strain, isolated from traditional Khiki cheese. Initially, its phenotypic characteristics, including resistance to harsh gastrointestinal conditions (low pH and bile salts) and its ability to colonize (surface hydrophobicity 58.9%, auto-aggregation 47.5%, and adhesion to Caco-2 cells 13.9%), were evaluated. The results showed that the cell-free supernatant (CFS) of this strain has strong antimicrobial effects against pathogens, particularly <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Typhi <i>(Salmonella</i> Typhi<i>).</i> CFS dose-dependently inhibited the formation of <i>Salmonella</i> Typhi biofilms by 79.52% (1/4×MIC) to 13.16% (4×MIC) and degraded mature biofilms, demonstrating anticancer activity against HeLa, HT-29, and MCF-7 cells (IC<sub>50</sub>: 63.6–79.7&#xa0;mg/mL). These anti-pathogenic effects were confirmed by observing morphological changes in the pathogen’s cells and a reduction in the expression of biofilm and invasion-related genes (<i>csgD</i>, <i>csgA</i>, <i>bcsA</i>, <i>invA</i>,&#xa0;and <i>invC</i>). Additionally, the strain demonstrated other health-promoting properties, including cholesterol reduction (54.62%) and high antioxidant activity. The safety assessments (no hemolytic or DNase activity and no biogenic amine production) and its ability to inhibit the proliferation of cancer cells were also favorable. In conclusion, due to its outstanding probiotic and anti-biofilm properties, the <i>L. acidophilus</i> TMPC 41127 strain is introduced as a promising candidate for food and therapeutic applications.</p>

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Probiotic potential of Lactobacillus acidophilus TMPC 41127 isolated from traditional cheese in inhibiting Salmonella Typhi biofilms and virulence genes

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月3日

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  • Behrooz Alizadeh Behbahani,
  • Parisa Ghasemi,
  • Alireza Vasiee,
  • Zeinab Mousavi

摘要

The present study comprehensively investigated the probiotic and anti-biofilm potential of the Lactobacillus acidophilus TMPC 41127 strain, isolated from traditional Khiki cheese. Initially, its phenotypic characteristics, including resistance to harsh gastrointestinal conditions (low pH and bile salts) and its ability to colonize (surface hydrophobicity 58.9%, auto-aggregation 47.5%, and adhesion to Caco-2 cells 13.9%), were evaluated. The results showed that the cell-free supernatant (CFS) of this strain has strong antimicrobial effects against pathogens, particularly Salmonella enterica subsp. enterica serovar Typhi (Salmonella Typhi). CFS dose-dependently inhibited the formation of Salmonella Typhi biofilms by 79.52% (1/4×MIC) to 13.16% (4×MIC) and degraded mature biofilms, demonstrating anticancer activity against HeLa, HT-29, and MCF-7 cells (IC50: 63.6–79.7 mg/mL). These anti-pathogenic effects were confirmed by observing morphological changes in the pathogen’s cells and a reduction in the expression of biofilm and invasion-related genes (csgD, csgA, bcsA, invA, and invC). Additionally, the strain demonstrated other health-promoting properties, including cholesterol reduction (54.62%) and high antioxidant activity. The safety assessments (no hemolytic or DNase activity and no biogenic amine production) and its ability to inhibit the proliferation of cancer cells were also favorable. In conclusion, due to its outstanding probiotic and anti-biofilm properties, the L. acidophilus TMPC 41127 strain is introduced as a promising candidate for food and therapeutic applications.