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<p>Although <i>Streptococcus bovis/equinus</i> complex (SBSEC) lysogens are prevalent in the rumen, studies on prophages experimentally induced from these strains are limited. Herein, we screened inducible prophages from ruminant-derived SBSEC strains, including the newly reported <i>S. ruminicola</i>, leading to the characterization of a representative temperate phage. We successfully induced a temperate phage (vB_SbS-proRumen) from <i>S. ruminicola</i> KCCM 90354, which has a broad host range, capable of lysing several SBSEC strains and other lactic acid bacteria. The vB_SbS-proRumen belongs to the Siphovirus, characterized by a short latent period, high burst size, and stability across pH and temperature conditions typical of subacute ruminal acidosis. The phage also demonstrates potent anti-biofilm activity. The vB_SbS-proRumen has a 38,092&#xa0;bp double-stranded DNA genome with 58 predicted open reading frames, sharing high similarity and conserved genetic organization with other previously predicted SBSEC prophages. This study provides valuable insights into the diversity and plasticity of SBSEC phages.</p>

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Characterization of a temperate bacteriophage isolated from the ruminant-derived Streptococcus bovis/equinus complex

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月17日

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  • Seon Young Park,
  • Jun-Seob Kim,
  • Do Young Jin,
  • Ye Bin Kim,
  • Tae Seon Cha,
  • Soojin Lim,
  • Seunghui Lee,
  • Seongwon Seo,
  • Ji Hyung Kim

摘要

Although Streptococcus bovis/equinus complex (SBSEC) lysogens are prevalent in the rumen, studies on prophages experimentally induced from these strains are limited. Herein, we screened inducible prophages from ruminant-derived SBSEC strains, including the newly reported S. ruminicola, leading to the characterization of a representative temperate phage. We successfully induced a temperate phage (vB_SbS-proRumen) from S. ruminicola KCCM 90354, which has a broad host range, capable of lysing several SBSEC strains and other lactic acid bacteria. The vB_SbS-proRumen belongs to the Siphovirus, characterized by a short latent period, high burst size, and stability across pH and temperature conditions typical of subacute ruminal acidosis. The phage also demonstrates potent anti-biofilm activity. The vB_SbS-proRumen has a 38,092 bp double-stranded DNA genome with 58 predicted open reading frames, sharing high similarity and conserved genetic organization with other previously predicted SBSEC prophages. This study provides valuable insights into the diversity and plasticity of SBSEC phages.