Scholarlink

<p>Vertebrates grow and mature with age after birth, deteriorate, and eventually die. However, the long lifespan of most vertebrates makes it challenging to identify markers of these distinct stages. Here, we leverage the short-lived vertebrate African Turquoise killifish (<i>N. furzeri</i>) to isolate molecular markers distinguishing gradual aging from late-onset aging. <i>N. furzeri</i> lifespan was divided into four stages–growth/maturation, young, midlife, and old–based on biological parameters. Cellular and molecular changes continually increasing or decreasing with age from young to midlife to old stages were defined as “gradual aging”, and changes specifically between midlife and old stages as “late-onset aging”. We discovered hepatic lipid droplets formed at birth and disappeared during the midlife stage. Metabolome and gene expression analyses of the liver where the majority of changes occurred identified several metabolites and genes as gradual aging markers (<i>e</i>.<i>g</i>., methylhistidine, glutarylcarnitine, and γ-butyrobetaine) and late-onset aging markers (<i>e</i>.<i>g</i>., creatine, homocitrulline, pipecolic acid, <i>p21</i>, <i>htra1</i>, and <i>slc13a5</i> genes) providing insights into alternative mechanisms of gradual aging and late-onset aging that may be conserved. Thus, molecular markers reflecting gradual aging and late-onset aging at the organ levels can be isolated using <i>N. furzeri</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification of gradual aging and late-onset aging markers using male African turquoise killifish

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月22日

查看全文
  • Junjie Chen,
  • Satoshi Kofuji,
  • Mizuki Kusaba,
  • Kota Abe,
  • Takehiko Sasaki,
  • Tohru Ishitani,
  • Hiroshi Nishina

摘要

Vertebrates grow and mature with age after birth, deteriorate, and eventually die. However, the long lifespan of most vertebrates makes it challenging to identify markers of these distinct stages. Here, we leverage the short-lived vertebrate African Turquoise killifish (N. furzeri) to isolate molecular markers distinguishing gradual aging from late-onset aging. N. furzeri lifespan was divided into four stages–growth/maturation, young, midlife, and old–based on biological parameters. Cellular and molecular changes continually increasing or decreasing with age from young to midlife to old stages were defined as “gradual aging”, and changes specifically between midlife and old stages as “late-onset aging”. We discovered hepatic lipid droplets formed at birth and disappeared during the midlife stage. Metabolome and gene expression analyses of the liver where the majority of changes occurred identified several metabolites and genes as gradual aging markers (e.g., methylhistidine, glutarylcarnitine, and γ-butyrobetaine) and late-onset aging markers (e.g., creatine, homocitrulline, pipecolic acid, p21, htra1, and slc13a5 genes) providing insights into alternative mechanisms of gradual aging and late-onset aging that may be conserved. Thus, molecular markers reflecting gradual aging and late-onset aging at the organ levels can be isolated using N. furzeri.