Scholarlink

<p>The clinical demand for paclitaxel necessitates novel strategies to enhance its production. While recent breakthroughs have elucidated the complete biosynthetic pathway in heterologous hosts, optimizing production within the native <i>Taxus</i> plant remains crucial. Here, we investigated the unexplored potential of using Tobacco rattle virus (TRV) as a controlled biotic elicitor in <i>Taxus baccata</i> seedlings. Using hydroponics and agroinfiltration, we found that TRV did not systemically infect or silence genes but triggered a localized hypersensitive response. Despite this, TRV significantly increased paclitaxel, baccatin III, and 10-deacetylbaccatin III levels in shoots, with the response depending on the infiltration method. Machine learning revealed exceptionally strong positive correlations among shoot taxoids (r &gt; 0.94) and identified baccatin III levels as the primary predictor of paclitaxel yield. Metabolic flux analysis quantified a preferential enhancement of early pathway steps and revealed a relative bottleneck in the final conversion to paclitaxel. These results demonstrate that TRV acts as a potent, growth-neutral biotic elicitor in <i>T. baccata</i>, where local defense couples with systemic metabolic reprogramming. Our integrated analysis establishes a novel virus-mediated strategy to complement pathway-engineering approaches for sustainable taxane production.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Tobacco rattle virus triggers local defense and systemically coordinates taxoid biosynthesis in Taxus baccata revealed by integrated metabolic analysis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月25日

查看全文
  • Seyed Ali Hosseini Tafreshi,
  • Mansour Shariati,
  • Mohammad Reza Mofid

摘要

The clinical demand for paclitaxel necessitates novel strategies to enhance its production. While recent breakthroughs have elucidated the complete biosynthetic pathway in heterologous hosts, optimizing production within the native Taxus plant remains crucial. Here, we investigated the unexplored potential of using Tobacco rattle virus (TRV) as a controlled biotic elicitor in Taxus baccata seedlings. Using hydroponics and agroinfiltration, we found that TRV did not systemically infect or silence genes but triggered a localized hypersensitive response. Despite this, TRV significantly increased paclitaxel, baccatin III, and 10-deacetylbaccatin III levels in shoots, with the response depending on the infiltration method. Machine learning revealed exceptionally strong positive correlations among shoot taxoids (r > 0.94) and identified baccatin III levels as the primary predictor of paclitaxel yield. Metabolic flux analysis quantified a preferential enhancement of early pathway steps and revealed a relative bottleneck in the final conversion to paclitaxel. These results demonstrate that TRV acts as a potent, growth-neutral biotic elicitor in T. baccata, where local defense couples with systemic metabolic reprogramming. Our integrated analysis establishes a novel virus-mediated strategy to complement pathway-engineering approaches for sustainable taxane production.