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<p>Spinal cord injury (SCI) remains a major clinical challenge due to the limited regenerative capacity of the central nervous system. This study presents the development and characterization of a gelatin–platelet-rich plasma (PRP) hydrogel as a therapeutic strategy for SCI repair. A composite gelatin–PRP scaffold was fabricated by incorporating PRP into a gelatin matrix to enhance tissue regeneration and functional recovery. In vitro analyses demonstrated excellent biocompatibility, with neuronal cell viability increasing from 75% on Day 1 to 96% on Day 7 (<i>p</i> &lt; 0.05). The release of growth factors including transforming growth factor β1 (TGF-β1), platelet-derived growth factor BB (PDGF-BB), and insulin-like growth factor 1 (IGF-1) followed a biphasic pattern, with approximately 85–88% released within 96&#xa0;h (<i>p</i> &lt; 0.05). In vivo, the PRP + Hydrogel group exhibited significantly improved locomotor recovery on the Basso–Beattie–Bresnahan (BBB) scale, along with reduced cavity formation, improved tissue preservation, and decreased scar-like tissue appearance compared with other treatments (n = 6 per group, <i>p</i> &lt; 0.05). These findings indicate that gelatin–PRP hydrogels support growth factor delivery, promote tissue repair, and improve functional recovery, highlighting their potential as a biomaterial platform for spinal cord repair.</p>

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Development and characterization of gelatin–platelet-rich plasma hydrogels for enhanced spinal cord injury repair

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月14日

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  • Shima Ghiasi Moradi,
  • Hesam-Uddin Hoseinzadeh,
  • Hamed Rad

摘要

Spinal cord injury (SCI) remains a major clinical challenge due to the limited regenerative capacity of the central nervous system. This study presents the development and characterization of a gelatin–platelet-rich plasma (PRP) hydrogel as a therapeutic strategy for SCI repair. A composite gelatin–PRP scaffold was fabricated by incorporating PRP into a gelatin matrix to enhance tissue regeneration and functional recovery. In vitro analyses demonstrated excellent biocompatibility, with neuronal cell viability increasing from 75% on Day 1 to 96% on Day 7 (p < 0.05). The release of growth factors including transforming growth factor β1 (TGF-β1), platelet-derived growth factor BB (PDGF-BB), and insulin-like growth factor 1 (IGF-1) followed a biphasic pattern, with approximately 85–88% released within 96 h (p < 0.05). In vivo, the PRP + Hydrogel group exhibited significantly improved locomotor recovery on the Basso–Beattie–Bresnahan (BBB) scale, along with reduced cavity formation, improved tissue preservation, and decreased scar-like tissue appearance compared with other treatments (n = 6 per group, p < 0.05). These findings indicate that gelatin–PRP hydrogels support growth factor delivery, promote tissue repair, and improve functional recovery, highlighting their potential as a biomaterial platform for spinal cord repair.