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<p><i>Ephedra alata</i> is a medicinal plant known for its rich composition of bioactive compounds and therapeutic properties. This study explores its phytochemical profile, antioxidant capacity, and potential anticancer effects. LC-MS analysis identified a variety of flavonoids and glycosylated derivatives, with quercetin 3-D-galactoside (28.49%) and 3,3’,4,4’,5,7-pentahydroxy-6-methoxyflavanonol-3-O-glucoside-5-O-glucuronic acid (10.74%) as the most abundant compounds. These flavonoids are recognized for their strong antioxidant and anticancer properties. The extract exhibited significant metal chelation activity, assessed using an EDTA-based calibration curve, indicating its potential to combat oxidative stress. Additionally, the ORAC assay confirmed a high free radical-scavenging ability, primarily linked to the presence of flavonoids such as quercetin, rutin, and kaempferol.To evaluate its anticancer potential, the methanolic extract was tested on liver cancer cell lines (Huh-7 and HepG2) using a WST-1 assay. The results showed a dose-dependent reduction in cell viability, with HepG2 cells displaying greater sensitivity than Huh-7 cells. Cytotoxicity extract’s IC₅₀ values exceeded 100&#xa0;µg/mL, indicating weak,&#xa0; cytotoxicity, the findings suggest that its bioactive compounds may play a role in inducing apoptosis and regulating oxidative stress in cancer cells. These results highlight <i>Ephedra alata</i> as a promising natural source of bioactive compounds with antioxidant potential, supporting further studies to explore its therapeutic applications.</p>

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LC–MS-based metabolomic characterization and POM pharmacophore analysis of the bioactive methanolic extract of Ephedra alata

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月7日

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  • Eman Ramadan Elsharkawy,
  • Salah Neghmouche Nacer,
  • Taibi Ben Hadda,
  • Faisal A. Almalki

摘要

Ephedra alata is a medicinal plant known for its rich composition of bioactive compounds and therapeutic properties. This study explores its phytochemical profile, antioxidant capacity, and potential anticancer effects. LC-MS analysis identified a variety of flavonoids and glycosylated derivatives, with quercetin 3-D-galactoside (28.49%) and 3,3’,4,4’,5,7-pentahydroxy-6-methoxyflavanonol-3-O-glucoside-5-O-glucuronic acid (10.74%) as the most abundant compounds. These flavonoids are recognized for their strong antioxidant and anticancer properties. The extract exhibited significant metal chelation activity, assessed using an EDTA-based calibration curve, indicating its potential to combat oxidative stress. Additionally, the ORAC assay confirmed a high free radical-scavenging ability, primarily linked to the presence of flavonoids such as quercetin, rutin, and kaempferol.To evaluate its anticancer potential, the methanolic extract was tested on liver cancer cell lines (Huh-7 and HepG2) using a WST-1 assay. The results showed a dose-dependent reduction in cell viability, with HepG2 cells displaying greater sensitivity than Huh-7 cells. Cytotoxicity extract’s IC₅₀ values exceeded 100 µg/mL, indicating weak,  cytotoxicity, the findings suggest that its bioactive compounds may play a role in inducing apoptosis and regulating oxidative stress in cancer cells. These results highlight Ephedra alata as a promising natural source of bioactive compounds with antioxidant potential, supporting further studies to explore its therapeutic applications.