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<p>Organoids are three-dimensional structures that develop in cultures of stem cells, and resemble multicellular organs. While performing long-term culture of <i>Taeniopygia guttata</i> (zebra finch) germline tissue, we observed the formation of germline organoids from cultures of both sexes. These macro-scale structures contain multiple cell types and retain Primordial Germ Cells (PGCs) and their descendant germ-cell lineages for two to four months of culture <i>in vitro</i>. We show that the PGC-specific germline-restricted chromosome (GRC) can be detected after three months of culture, and that the organoids exhibit DAZL- and DDX4-positive structures resembling germinal epithelia. Taken together our results open new possibilities for the study of key steps of avian reproductive development.</p>

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Germline organoids develop in vitro from embryonic Taeniopygia guttata (zebra finch) cultures

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月20日

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  • Bianca Brown,
  • Elizabeth Nagy,
  • Ajuni K. Takkar,
  • Naomi A. Greengold,
  • Shreyas S. Gujar,
  • Ali Amini,
  • Mary A. Collins,
  • Javad Rajabi,
  • Jenna Walls,
  • Chenai Kaminski,
  • Michael Sanchez,
  • Elle McQuire-Guzman,
  • Taraji Ellington,
  • John R. Bracht

摘要

Organoids are three-dimensional structures that develop in cultures of stem cells, and resemble multicellular organs. While performing long-term culture of Taeniopygia guttata (zebra finch) germline tissue, we observed the formation of germline organoids from cultures of both sexes. These macro-scale structures contain multiple cell types and retain Primordial Germ Cells (PGCs) and their descendant germ-cell lineages for two to four months of culture in vitro. We show that the PGC-specific germline-restricted chromosome (GRC) can be detected after three months of culture, and that the organoids exhibit DAZL- and DDX4-positive structures resembling germinal epithelia. Taken together our results open new possibilities for the study of key steps of avian reproductive development.