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<p>Ventilator-induced myocardial injury is a common complication in clinical practice during mechanical ventilation in critically ill patients. To date, the changes in myocardial mRNA expression in rat models of ventilator-induced myocardial injury have not been elucidated. This study aims to clarify these expression changes. HE staining revealed inflammatory cell infiltration and disordered myocardial fiber arrangement in the myocardial tissues. mRNA sequencing results showed 428 significantly differentially expressed (DE) mRNAs in the M6h group, with 134 upregulated and 294 downregulated. In the M12h group, 550 DE mRNAs were identified, with 216 upregulated and 334 downregulated. Gene Ontology (GO) analysis indicated that these mRNAs are involved in biological processes and pathways such as signal transduction, protein binding, and metal ion binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed the most relevant signaling pathway, namely the mitogen-activated protein kinase(MAPK) pathway. Changes in genes such as complement C4B(C4B), thioredoxin interacting protein (TXNIP), and JunD proto-oncogene (JUND) were particularly significant and may play an important regulatory role. This study identified the myocardial mRNA expression profile in rats with myocardial injury following mechanical ventilation using high-throughput mRNA sequencing technology, providing new perspectives for elucidating the key mechanisms of ventilator-induced myocardial injury. </p>

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Changes in mRNA expression in the myocardium of rats with ventilator-induced myocardial injury

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月2日

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  • Shibo Liao,
  • Dong Zhao,
  • Longhuan Zeng,
  • Shihan Zhou,
  • Hanqiao Xu,
  • Yongke Zheng

摘要

Ventilator-induced myocardial injury is a common complication in clinical practice during mechanical ventilation in critically ill patients. To date, the changes in myocardial mRNA expression in rat models of ventilator-induced myocardial injury have not been elucidated. This study aims to clarify these expression changes. HE staining revealed inflammatory cell infiltration and disordered myocardial fiber arrangement in the myocardial tissues. mRNA sequencing results showed 428 significantly differentially expressed (DE) mRNAs in the M6h group, with 134 upregulated and 294 downregulated. In the M12h group, 550 DE mRNAs were identified, with 216 upregulated and 334 downregulated. Gene Ontology (GO) analysis indicated that these mRNAs are involved in biological processes and pathways such as signal transduction, protein binding, and metal ion binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed the most relevant signaling pathway, namely the mitogen-activated protein kinase(MAPK) pathway. Changes in genes such as complement C4B(C4B), thioredoxin interacting protein (TXNIP), and JunD proto-oncogene (JUND) were particularly significant and may play an important regulatory role. This study identified the myocardial mRNA expression profile in rats with myocardial injury following mechanical ventilation using high-throughput mRNA sequencing technology, providing new perspectives for elucidating the key mechanisms of ventilator-induced myocardial injury.