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<p>We present a dual-wavelength, multi-parametric Interferometric Reflectance Imaging Sensor (MP-IRIS) for real-time, label free, multiplexed binding kinetic measurements without the “bulk-effect”. Our platform dynamically tracks the conditions of the solution and corrects in real time, demonstrating a reduction in bulk effect to 3 pg/mm<InlineEquation ID="IEq1"> <EquationSource Format="TEX">\(\vphantom{0}^2\)</EquationSource> </InlineEquation> with bulk refractive index change of <InlineEquation ID="IEq2"> <EquationSource Format="TEX">\(10^{-3}\)</EquationSource> </InlineEquation> RIU. By incorporating multi-wavelength data acquisition, we improve the repeatability and reduce quantification error due to variation in chip manufacturing and surface treatment. Proof-of-concept DNA hybridization assays validate accurate, multiplexed binding measurements under diverse chip conditions. MP-IRIS retains the high sensitivity of prior IRIS designs, lowering the overall complexity of the system and offering potential applications in small-molecule analysis.</p>

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Multi-parametric interferometric reflectance imaging sensor

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月28日

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  • Mete Aslan,
  • Stephen Snekvik,
  • Elif Seymour,
  • Sinem Diken Gür,
  • Eren Ergene,
  • Nese Lortlar Ünlü,
  • M. Selim Ünlü

摘要

We present a dual-wavelength, multi-parametric Interferometric Reflectance Imaging Sensor (MP-IRIS) for real-time, label free, multiplexed binding kinetic measurements without the “bulk-effect”. Our platform dynamically tracks the conditions of the solution and corrects in real time, demonstrating a reduction in bulk effect to 3 pg/mm \(\vphantom{0}^2\) with bulk refractive index change of \(10^{-3}\) RIU. By incorporating multi-wavelength data acquisition, we improve the repeatability and reduce quantification error due to variation in chip manufacturing and surface treatment. Proof-of-concept DNA hybridization assays validate accurate, multiplexed binding measurements under diverse chip conditions. MP-IRIS retains the high sensitivity of prior IRIS designs, lowering the overall complexity of the system and offering potential applications in small-molecule analysis.