<p>The illegal poaching of rhinoceros for their horns continues to threaten the survival of the species despite ongoing conservation efforts. As black-market demand grows, poaching and smuggling methods have become more advanced, outpacing the effectiveness of current strategies. In response, the Rhisotope Project proposes a novel deterrent: the implantation of low-activity radioactive sources into rhinoceros’ horns to exploit the public fear of radiation and improve horn detection at borders. This research shows that radioactive horn implantation poses minimal risk to the animals’ health and that the probability of radiation-induced harm is significantly lower than the likelihood of being poached. Measurements collected from 16 white rhinoceroses six months post-implantation revealed no adverse biological effects, and all recorded dose rates remained below deterministic thresholds. These findings support both the safety and feasibility of the approach. Radiation inclusion in the horns could decrease poaching risk, improve seizure rates, and support rhinoceros population survival. This method may also serve as a reference point for safe radiation application in the other four rhinoceros’ species. Additionally, the concept could extend to other endangered fauna and flora. This innovative approach shows the ethical and practical utilization of radiation-based anti-poaching measures, contributing to conservation efforts.</p>