<p>The drought resistance of <i>Cinnamomum migao</i> seedlings inoculated with endophytic fungi was explored under different drought stress conditions. The seedlings of <i>C. migao</i> were inoculated with <i>Trichoderma harzianum</i> and <i>Trichoderma longibrachiatum</i> in the rhizosphere, with non-inoculated seedlings serving as the control. Then, the seedlings of <i>C. migao</i> were exposed to different drought stress gradients: normal water supply (80 ± 5% moisture, control), mild (60 ± 5% moisture, T1), moderate (45 ± 5% moisture, T2), and severe (25 ± 5% moisture, T3) drought stress. After 30&#xa0;days of each drought stress treatment, samples were collected, and growth parameters, osmotic substance content, antioxidant enzyme activity, chlorophyll content, and endogenous hormone concentrations were measured. Compared with the control group, inoculation with <i>T. harzianum</i> and <i>T. longibrachiatum</i> promoted the growth of <i>C. migao</i> seedlings to varying extents. This inoculation also enhanced their root absorption capacity, increased the relative water content, chlorophyll, and abscisic acid (ABA) levels in leaf tissues (<i>p</i> &lt; 0.05), and facilitated the accumulation of osmotic substances while boosting antioxidant enzyme activity (<i>p</i> &lt; 0.05). Furthermore, it reduced water potential and decreased the contents of malonaldehyde (MDA), indoleacetic acid (IAA), gibberellin (GA₃), and ribosylzeatin (ZR) (<i>p</i> &lt; 0.05). <i>T. harzianum</i> and <i>T. longibrachiatum</i> significantly improved the drought resistance of <i>C. migao</i> seedlings under stress conditions. These findings provide a theoretical basis for further research and development of microbial inoculants.</p>

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Two Trichoderma endophytic fungi alleviates drought stress and improves plant growth in Cinnamomum migao seedlings

  • Lan-yue Zhang,
  • Tao Fu,
  • Jing-zhong Chen,
  • Shuang-yan Hu

摘要

The drought resistance of Cinnamomum migao seedlings inoculated with endophytic fungi was explored under different drought stress conditions. The seedlings of C. migao were inoculated with Trichoderma harzianum and Trichoderma longibrachiatum in the rhizosphere, with non-inoculated seedlings serving as the control. Then, the seedlings of C. migao were exposed to different drought stress gradients: normal water supply (80 ± 5% moisture, control), mild (60 ± 5% moisture, T1), moderate (45 ± 5% moisture, T2), and severe (25 ± 5% moisture, T3) drought stress. After 30 days of each drought stress treatment, samples were collected, and growth parameters, osmotic substance content, antioxidant enzyme activity, chlorophyll content, and endogenous hormone concentrations were measured. Compared with the control group, inoculation with T. harzianum and T. longibrachiatum promoted the growth of C. migao seedlings to varying extents. This inoculation also enhanced their root absorption capacity, increased the relative water content, chlorophyll, and abscisic acid (ABA) levels in leaf tissues (p < 0.05), and facilitated the accumulation of osmotic substances while boosting antioxidant enzyme activity (p < 0.05). Furthermore, it reduced water potential and decreased the contents of malonaldehyde (MDA), indoleacetic acid (IAA), gibberellin (GA₃), and ribosylzeatin (ZR) (p < 0.05). T. harzianum and T. longibrachiatum significantly improved the drought resistance of C. migao seedlings under stress conditions. These findings provide a theoretical basis for further research and development of microbial inoculants.