Scholarlink

<p>We present a spatial transcriptomic map of the mouse urinary bladder at near single-cell resolution using Visium HD technology with 8 × 8&#xa0;μm bins. After quality filtering, we analyzed 66,471 bins and 9,364 genes. Cluster annotation was supported by canonical marker genes and reference datasets, including Tabula Muris and Mouse Cell Atlas. Thirteen distinct clusters corresponding to major bladder compartments—the urothelium, lamina propria, and smooth muscle—were identified. Gene expression patterns revealed gradual transitions across tissue layers rather than sharp boundaries. Within the urothelium, basal, intermediate, and umbrella cells were distinguished, with umbrella cells showing potentially polarized mRNA distribution. The lamina propria primarily consisted of fibroblasts and a small number of immune cells. Four smooth muscle clusters were detected, contrasting with the single cluster typically identified by non-spatial single-cell RNA sequencing. This spatially resolved transcriptome map highlights the advantages of spatial transcriptomics in complementing traditional histology and scRNA-seq. Our map provides a valuable resource for advancing understanding of mouse urinary bladder biology and establishes a foundation for future studies of bladder pathology.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Spatial transcriptomic map of the mouse urinary bladder

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月16日

查看全文
  • Nives Matković,
  • Andrea Gelemanović,
  • Kristijana Popović,
  • Katarina Vilović,
  • Caterina Oneto,
  • Dejan Lazarević,
  • Ivana Marinović Terzić,
  • Ivana Novak,
  • Jelena Korac-Prlic,
  • Blanka Milić Roje,
  • Janoš Terzić

摘要

We present a spatial transcriptomic map of the mouse urinary bladder at near single-cell resolution using Visium HD technology with 8 × 8 μm bins. After quality filtering, we analyzed 66,471 bins and 9,364 genes. Cluster annotation was supported by canonical marker genes and reference datasets, including Tabula Muris and Mouse Cell Atlas. Thirteen distinct clusters corresponding to major bladder compartments—the urothelium, lamina propria, and smooth muscle—were identified. Gene expression patterns revealed gradual transitions across tissue layers rather than sharp boundaries. Within the urothelium, basal, intermediate, and umbrella cells were distinguished, with umbrella cells showing potentially polarized mRNA distribution. The lamina propria primarily consisted of fibroblasts and a small number of immune cells. Four smooth muscle clusters were detected, contrasting with the single cluster typically identified by non-spatial single-cell RNA sequencing. This spatially resolved transcriptome map highlights the advantages of spatial transcriptomics in complementing traditional histology and scRNA-seq. Our map provides a valuable resource for advancing understanding of mouse urinary bladder biology and establishes a foundation for future studies of bladder pathology.