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<p>In this study, endospore-forming bacteria were isolated, screened, and characterized from samples collected in Tan Phu protection forest for potential use in biofertilizer application. Twelve endospore-forming bacteria showed the ability to produce indole-3-acetic acid were evaluated. Based on the relative enzyme activity, siderophore production, phosphate and potassium solubilization, strain CN12 was selected. Strain CN12 was identified as <i>Bacillus amyloliquefaciens</i> based on 16&#xa0;S rRNA gene sequencing. The liquid culture medium using inexpensive substrates was optimized for increasing its endospore production. The medium consisted of 10% mung bean sprouts extract solution, 0.87% molasses, 0.12% urea, and 0.06% MgSO<sub>4</sub> produced the spore yield of 5.53 ± 3.5 (× 10<sup>8</sup> CFU/mL) for strain CN12. Malabar spinach plants treated with strain CN12 showed significantly increases of 39.4 ± 8.9 to 77.6 ± 11.8&#xa0;g in fresh weight, 17.1 ± 3.0 to 65.8 ± 17.2&#xa0;cm in plant height, and 2.9 ± 0.9 to 6.8 ± 2.3&#xa0;g in root weight compared to the control. These observations revealed that strain CN12 is a promising candidate for biofertilizer production.</p>

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Cost-effective plant-based medium for enhanced spore production of B. amyloliquefaciens CN12 for biofertilizer application

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月5日

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  • Tuan Ngoc Nguyen,
  • Tu Cam Ly,
  • Nghi Tran,
  • Nhung Hong Nguyen,
  • Rudolf Kiefer,
  • Thu Thien Pham,
  • Hien My Tran,
  • Hung Phi Nguyen,
  • Thuan Phuoc Dinh

摘要

In this study, endospore-forming bacteria were isolated, screened, and characterized from samples collected in Tan Phu protection forest for potential use in biofertilizer application. Twelve endospore-forming bacteria showed the ability to produce indole-3-acetic acid were evaluated. Based on the relative enzyme activity, siderophore production, phosphate and potassium solubilization, strain CN12 was selected. Strain CN12 was identified as Bacillus amyloliquefaciens based on 16 S rRNA gene sequencing. The liquid culture medium using inexpensive substrates was optimized for increasing its endospore production. The medium consisted of 10% mung bean sprouts extract solution, 0.87% molasses, 0.12% urea, and 0.06% MgSO4 produced the spore yield of 5.53 ± 3.5 (× 108 CFU/mL) for strain CN12. Malabar spinach plants treated with strain CN12 showed significantly increases of 39.4 ± 8.9 to 77.6 ± 11.8 g in fresh weight, 17.1 ± 3.0 to 65.8 ± 17.2 cm in plant height, and 2.9 ± 0.9 to 6.8 ± 2.3 g in root weight compared to the control. These observations revealed that strain CN12 is a promising candidate for biofertilizer production.