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<p>Soil salinization threatens agricultural development, and understanding the alkali tolerance mechanism of barley is crucial for breeding. Using the alkali-tolerant barley variety Jia-HY460-6R and the alkali-sensitive variety Noire Maroc as materials, morphological and physiological indicators were determined and proteomic analysis was conducted after treatment with 100 mmol·L⁻¹ Na<sub>2</sub>CO<sub>3</sub>:NaHCO<sub>3</sub>. The results showed thatJia-HY460-6R exhibited superior performance compared to Noire Maroc in terms of growth indicators, antioxidant capacity, ion balance, and osmotic regulation. A total of 1359 differentially abundant proteins were identified, and 27 key proteins were screened, involving multiple metabolic pathways such as the MAPK signaling pathway. This study provides a basis for understanding the molecular mechanism of barley alkali tolerance and breeding.</p>

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Physiological responses and proteomic analysis of different barley varieties under alkaline stress

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月2日

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  • Zhe Li,
  • Jianbo Li,
  • Yanan Jin,
  • Tu Ba,
  • Ersuo Lv,
  • Zhiping Liu,
  • Shoujun Xu

摘要

Soil salinization threatens agricultural development, and understanding the alkali tolerance mechanism of barley is crucial for breeding. Using the alkali-tolerant barley variety Jia-HY460-6R and the alkali-sensitive variety Noire Maroc as materials, morphological and physiological indicators were determined and proteomic analysis was conducted after treatment with 100 mmol·L⁻¹ Na2CO3:NaHCO3. The results showed thatJia-HY460-6R exhibited superior performance compared to Noire Maroc in terms of growth indicators, antioxidant capacity, ion balance, and osmotic regulation. A total of 1359 differentially abundant proteins were identified, and 27 key proteins were screened, involving multiple metabolic pathways such as the MAPK signaling pathway. This study provides a basis for understanding the molecular mechanism of barley alkali tolerance and breeding.