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<p>Marine yeasts are underexplored resource for bioactive polysaccharides with potential pharmaceutical activities. In this study, five marine yeast isolates from mangrove shrubs were screened for β-glucan production. Isolate Y<sub>4</sub> exhibited the highest β-glucan yield, reaching 17.11% of cell fresh weight- approximately 1.57-fold higher than <i>Saccharomyces cerevisiae</i>. Molecular identification revealed Y<sub>4</sub> to be <i>Candida tropicalis</i> AUMC 15,533 through ITS rRNA sequencing and phylogenetic analysis. Structural characterization using ¹H NMR and FT-IR confirmed the presence of β-1,3 and β-1,6 glucan linkages. Optimization of β-glucan production via Plackett–Burman design identified glucose, yeast extract, shaking speed, and incubation time as critical factors, achieving a maximum yield of 0.42&#xa0;g/100 mL. The extracted β-glucan demonstrated significant biological activities, including antioxidant capacity (IC₅₀= 18.31&#xa0;µg/mL), wound healing efficacy (80.19% closure in 24&#xa0;h), and anti-inflammatory effects, as evidenced by reduced expression of pro-inflammatory cytokines TNF-α (74.5% reduction) and IL-6 (80.8% reduction). Carboxymethylated β-glucan showed lower but still substantial activity across all assays. These findings highlight the superior biosynthetic capacity of marine <i>C. tropicalis</i> for β-glucan production and its potential therapeutic properties. This study underscores the importance of exploring marine microbial diversity for potential bioactive molecules with biomedical applications.</p>

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Wound-healing, anti-inflammatory, and antioxidant activities of β-glucan from red sea-mangroves-associated Candida tropicalis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月1日

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  • Aya T. ElGazzare,
  • Basma M. Alkersh,
  • Soraya A. Sabry,
  • Hanan A. Ghozlan

摘要

Marine yeasts are underexplored resource for bioactive polysaccharides with potential pharmaceutical activities. In this study, five marine yeast isolates from mangrove shrubs were screened for β-glucan production. Isolate Y4 exhibited the highest β-glucan yield, reaching 17.11% of cell fresh weight- approximately 1.57-fold higher than Saccharomyces cerevisiae. Molecular identification revealed Y4 to be Candida tropicalis AUMC 15,533 through ITS rRNA sequencing and phylogenetic analysis. Structural characterization using ¹H NMR and FT-IR confirmed the presence of β-1,3 and β-1,6 glucan linkages. Optimization of β-glucan production via Plackett–Burman design identified glucose, yeast extract, shaking speed, and incubation time as critical factors, achieving a maximum yield of 0.42 g/100 mL. The extracted β-glucan demonstrated significant biological activities, including antioxidant capacity (IC₅₀= 18.31 µg/mL), wound healing efficacy (80.19% closure in 24 h), and anti-inflammatory effects, as evidenced by reduced expression of pro-inflammatory cytokines TNF-α (74.5% reduction) and IL-6 (80.8% reduction). Carboxymethylated β-glucan showed lower but still substantial activity across all assays. These findings highlight the superior biosynthetic capacity of marine C. tropicalis for β-glucan production and its potential therapeutic properties. This study underscores the importance of exploring marine microbial diversity for potential bioactive molecules with biomedical applications.