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<p>Previous studies in mice have reported that melanin-concentrating hormone (MCH) regulates bone calcification, an essential process in bone regeneration. However, the mechanisms by which MCH influences the activities of bone cells involved in calcification remain unclear. Goldfish scales, like mammalian bone, consist of osteoblasts, osteoclasts, and a calcified matrix, making regenerating goldfish scales a useful model for studying bone regeneration. In the present study, a single MCH treatment suppressed the expression of genes encoding proteins associated with osteoclast activity in cultured goldfish scales. Moreover, intraperitoneal administration of MCH decreased plasma calcium concentrations and tartrate-resistant acid phosphatase (TRAP) activity, an osteoclast activity marker, in goldfish 24&#xa0;h after administration. Gene expression analysis suggested that MCH receptor 2 is involved in MCH-mediated regulation of calcification in goldfish scales. In contrast, continuous intraperitoneal administration of MCH elevated both alkaline phosphatase, an osteoblast marker, and TRAP activities in regenerating scales and resulted in decreased scale calcium levels. These results indicate that a single MCH treatment inhibits osteoclast activity in goldfish scales, leading to reduced plasma calcium levels, whereas prolonged MCH treatment affects both osteoclasts and osteoblasts in regenerating scales, thereby resulting in decreased calcium contents in the scales.</p>

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Melanin concentrating hormone regulates bone cell activities and calcium metabolism in regenerating goldfish scales

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月20日

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  • Kohei Kuroda,
  • Satsuki Kimura,
  • Kanta Mizusawa,
  • Akiyoshi Takahashi,
  • Yusuke Maruyama,
  • Yoshiaki Tabuchi,
  • Yukihiro Furusawa,
  • Tasuku Hirose,
  • Kaito Hatano,
  • Akihiro Sakatoku,
  • Masato Honda,
  • Hajime Matsubara,
  • Hiroyuki Mishima,
  • Naruto Yoshida,
  • Ajai K. Srivastav,
  • Jun Hirayama,
  • Atsuhiko Hattori,
  • Nobuo Suzuki

摘要

Previous studies in mice have reported that melanin-concentrating hormone (MCH) regulates bone calcification, an essential process in bone regeneration. However, the mechanisms by which MCH influences the activities of bone cells involved in calcification remain unclear. Goldfish scales, like mammalian bone, consist of osteoblasts, osteoclasts, and a calcified matrix, making regenerating goldfish scales a useful model for studying bone regeneration. In the present study, a single MCH treatment suppressed the expression of genes encoding proteins associated with osteoclast activity in cultured goldfish scales. Moreover, intraperitoneal administration of MCH decreased plasma calcium concentrations and tartrate-resistant acid phosphatase (TRAP) activity, an osteoclast activity marker, in goldfish 24 h after administration. Gene expression analysis suggested that MCH receptor 2 is involved in MCH-mediated regulation of calcification in goldfish scales. In contrast, continuous intraperitoneal administration of MCH elevated both alkaline phosphatase, an osteoblast marker, and TRAP activities in regenerating scales and resulted in decreased scale calcium levels. These results indicate that a single MCH treatment inhibits osteoclast activity in goldfish scales, leading to reduced plasma calcium levels, whereas prolonged MCH treatment affects both osteoclasts and osteoblasts in regenerating scales, thereby resulting in decreased calcium contents in the scales.