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<p>The Oriental fruit fly, <i>Bactrocera dorsalis</i> (Diptera: Tephritidae), is a globally significant agricultural pest necessitating novel and sustainable management strategies. This study investigated the efficacy of two stable bio-control agents derived from the naturally associated mycobiome of <i>Zaprionus indianus</i>: the yeast species <i>Debaryomyces hansenii</i> ziha1 and <i>Pichia kudriavzevii</i> zibd3, formulated with Essential Oil of Citral (EOC). Efficacy was evaluated via stability testing across 7&#xa0;°C, 25&#xa0;°C and 35&#xa0;°C, compatibility assessment (T-factor), behavioral preference assays, insecticidal trials against first-instar larvae, and chemical characterization using GC–MS and Y-tube olfactometry. Both EOC-based formulations demonstrated high stability and acceptable compatibility with the fungi (EOC ≤ 1%, T ≈ 55), but exhibited strikingly opposite behavioral effects on adult <i>B. dorsalis</i>. <i>D. hansenii</i>-EOC showed significant attractant activity (− 95 ± 0.5%), which was correlated with VOCs such as Acetophenone and Hexadecenal, while <i>P. kudriavzevii</i>-EOC acted as a potent repellent (100 ± 0.5%), attributed to compounds including <i>p</i>-Mentha1,5-dien-8-ol and Naphthalene. Furthermore, <i>P. kudriavzevii</i>-EOC exhibited a synergistic lethal effect against pre-adult stages, causing over 50% larval mortality. In conclusion, the insect mycobiome serves as a rich and selective source for chemical ecology-based pest management, offering stable, dual-action bio-formulations (Attractant and Repellent/Larvicide) that are ideal for developing targeted “Push–Pull” or “Attract-and-Kill” strategies in <i>B. dorsalis</i> integrated pest management (IPM) programs.</p>

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Behavioral and lethal effects of yeast based bioformulations on Bactrocera dorsalis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月13日

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  • Seema Ramniwas,
  • Aanchal Sharma,
  • Narendra Vikram Singh,
  • Girish Kumar

摘要

The Oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), is a globally significant agricultural pest necessitating novel and sustainable management strategies. This study investigated the efficacy of two stable bio-control agents derived from the naturally associated mycobiome of Zaprionus indianus: the yeast species Debaryomyces hansenii ziha1 and Pichia kudriavzevii zibd3, formulated with Essential Oil of Citral (EOC). Efficacy was evaluated via stability testing across 7 °C, 25 °C and 35 °C, compatibility assessment (T-factor), behavioral preference assays, insecticidal trials against first-instar larvae, and chemical characterization using GC–MS and Y-tube olfactometry. Both EOC-based formulations demonstrated high stability and acceptable compatibility with the fungi (EOC ≤ 1%, T ≈ 55), but exhibited strikingly opposite behavioral effects on adult B. dorsalis. D. hansenii-EOC showed significant attractant activity (− 95 ± 0.5%), which was correlated with VOCs such as Acetophenone and Hexadecenal, while P. kudriavzevii-EOC acted as a potent repellent (100 ± 0.5%), attributed to compounds including p-Mentha1,5-dien-8-ol and Naphthalene. Furthermore, P. kudriavzevii-EOC exhibited a synergistic lethal effect against pre-adult stages, causing over 50% larval mortality. In conclusion, the insect mycobiome serves as a rich and selective source for chemical ecology-based pest management, offering stable, dual-action bio-formulations (Attractant and Repellent/Larvicide) that are ideal for developing targeted “Push–Pull” or “Attract-and-Kill” strategies in B. dorsalis integrated pest management (IPM) programs.