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<p>Type 1 diabetes mellitus (T1DM) can lead to severe diabetic osteopathy, largely driven by alterations in the bone marrow hypoxic microenvironment and damage to type H vessels. This study employed multimodal imaging—including DCE-MRI, Micro-CT, and USPIO-enhanced MRI—to enable early in vivo assessment of hypoxic changes and type H vessel impairment in a T1DM rabbit model. Experiments involved 20 rabbits with alloxan-induced T1DM and controls, evaluated four months post-modeling. Imaging revealed significant differences in bone marrow microcirculatory perfusion and vascular permeability in T1DM rabbits, along with elevated USPIO uptake and regional heterogeneity that correlated with type H vessel distribution. Accompanying pathological changes were confirmed via immunofluorescence, qPCR, and transmission electron microscopy, suggesting an association of the AGEs/ROS-HIF-1α-VEGF pathway with these microvascular lesions. Our findings offer visual and quantitative imaging evidence to inform future clinical strategies targeting type H vessel hypoxia in diabetic osteopathy.</p>

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Multimodal imaging evaluation of hypoxic bone marrow microenvironment and type H vascular injury in diabetes

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月4日

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  • Haoran Lei,
  • Kejun Wang,
  • Liang Li,
  • Changsheng Liu,
  • Yunfei Zha

摘要

Type 1 diabetes mellitus (T1DM) can lead to severe diabetic osteopathy, largely driven by alterations in the bone marrow hypoxic microenvironment and damage to type H vessels. This study employed multimodal imaging—including DCE-MRI, Micro-CT, and USPIO-enhanced MRI—to enable early in vivo assessment of hypoxic changes and type H vessel impairment in a T1DM rabbit model. Experiments involved 20 rabbits with alloxan-induced T1DM and controls, evaluated four months post-modeling. Imaging revealed significant differences in bone marrow microcirculatory perfusion and vascular permeability in T1DM rabbits, along with elevated USPIO uptake and regional heterogeneity that correlated with type H vessel distribution. Accompanying pathological changes were confirmed via immunofluorescence, qPCR, and transmission electron microscopy, suggesting an association of the AGEs/ROS-HIF-1α-VEGF pathway with these microvascular lesions. Our findings offer visual and quantitative imaging evidence to inform future clinical strategies targeting type H vessel hypoxia in diabetic osteopathy.