Scholarlink

<p><i>Bacillus amyloliquefaciens</i> strain NJF-55, which was isolated from the distal colon of a healthy ram, was investigated as a probiotic candidate. We produced a high-quality assembly comprising a single circular chromosome (3,854,703&#xa0;bp; 46.1% GC) and a small plasmid (6033&#xa0;bp), and reconciled multi pipeline annotations to derive a consensus feature set. Functional profiling mapped 2334 genes to 2027 KEGG orthologs (1835 unique), indicating complete central metabolism, broad transport capacity, and stress-adaptation pathways; 94 CAZymes supported glycan turnover and cell-envelope remodeling. Secondary-metabolite mining revealed hallmark <i>Bacillus</i> BGCs—surfactin, fengycin, bacillaene, and macrolactin—and flagged putative novel compounds (a PKS-like region with ~ 7% similarity to the butirosin A/B reference); Five bacteriocin/RiPP regions were identified (class IV lanthipeptide, amylocyclicin, ComX2 pheromone, UviB-like and colicin-like). Genome-safety screening detected no classical virulence determinants or acquired clinically relevant AMR genes; four intact prophages lacked toxin/AMR cargo, and the plasmid encoded no risk loci. Comparative genomics with four reference <i>bacilli</i> yielded a 5,398-cluster pangenome; NJF-55 retained 325 probiotic-associated clusters (largely core) and showed 30 copy-number expansions, notably in the NRPS/PKS and DNA replication/repair families, consistent with a “core-driven, dosage-tuned” architecture. These features suggest that NJF-55 may represent a safe and robust candidate for ruminant gut applications, while underscoring the need for targeted metabolomics and controlled <i>in vivo</i> validation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Complete genome sequence and functional characterization of Bacillus amyloliquefaciens NJF-55: a sheep-derived probiotic candidate

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月27日

查看全文
  • Baraa Akeel Al-Hasan,
  • Ali H. D. Janabi,
  • Carina Helmer

摘要

Bacillus amyloliquefaciens strain NJF-55, which was isolated from the distal colon of a healthy ram, was investigated as a probiotic candidate. We produced a high-quality assembly comprising a single circular chromosome (3,854,703 bp; 46.1% GC) and a small plasmid (6033 bp), and reconciled multi pipeline annotations to derive a consensus feature set. Functional profiling mapped 2334 genes to 2027 KEGG orthologs (1835 unique), indicating complete central metabolism, broad transport capacity, and stress-adaptation pathways; 94 CAZymes supported glycan turnover and cell-envelope remodeling. Secondary-metabolite mining revealed hallmark Bacillus BGCs—surfactin, fengycin, bacillaene, and macrolactin—and flagged putative novel compounds (a PKS-like region with ~ 7% similarity to the butirosin A/B reference); Five bacteriocin/RiPP regions were identified (class IV lanthipeptide, amylocyclicin, ComX2 pheromone, UviB-like and colicin-like). Genome-safety screening detected no classical virulence determinants or acquired clinically relevant AMR genes; four intact prophages lacked toxin/AMR cargo, and the plasmid encoded no risk loci. Comparative genomics with four reference bacilli yielded a 5,398-cluster pangenome; NJF-55 retained 325 probiotic-associated clusters (largely core) and showed 30 copy-number expansions, notably in the NRPS/PKS and DNA replication/repair families, consistent with a “core-driven, dosage-tuned” architecture. These features suggest that NJF-55 may represent a safe and robust candidate for ruminant gut applications, while underscoring the need for targeted metabolomics and controlled in vivo validation.