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<p>Cardiomyocyte differentiation is a complex process involving significant metabolic remodeling, but its impact on cellular redox state and cell damage remains poorly understood. Using metabolomics, biophysical, and biochemical approaches, we characterized, in vitro, the metabolic shift of differentiating cardiomyocytes and its implications for oxidative damage. We found that differentiating cardiomyocytes undergo a broad metabolic reprogramming from a glycolytic to an oxidative state, marked by increased activity in key pathways, including malate-aspartate shuttle, glutathione metabolism, and tricarboxylic acid cycle. This metabolic transition was associated with mitochondrial enlargement and increased reactive oxygen species (ROS) production. Intriguingly, despite ROS increase, differentiated cells maintained similar levels of DNA damage as cardiomyoblasts and were more resistant to a H₂O₂ challenge. Our findings suggest that metabolic adaptations during cardiomyocyte differentiation enhance their capacity to mitigate oxidative stress damage, providing an adaptive avenue that enables cardiomyocyte survival upon exposure to an oxygen-rich environment.</p>

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Metabolic reprogramming enhances oxidative stress resistance in differentiating cardiomyocytes

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月20日

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  • Lara Basseres Novais,
  • Beatriz Rocha Ilidio Rodrigues,
  • Flávia Oliveira Borges Pereira,
  • Alan Gonçalves Amaral,
  • Sofya Castilho Lapa,
  • Lucas Lopes Maldonado,
  • Pedro Víctor-Carvalho,
  • Isabela Aparecida Moretto,
  • Hans Rolando Zamora-Obando,
  • Mariana Conceição da Silva,
  • Ana Paula Samogim,
  • Ingridi Rafaela de Brito,
  • Maria das Graças de Souza Carvalho,
  • Antonio Thiago Pereira Campos,
  • Michelle Bueno de Moura Pereira Antunes,
  • Carlos Lenz Cesar,
  • Hernandes F. Carvalho,
  • Ana Valéria Colnaghi Simionato,
  • André Alexandre de Thomaz,
  • Aline Mara dos Santos

摘要

Cardiomyocyte differentiation is a complex process involving significant metabolic remodeling, but its impact on cellular redox state and cell damage remains poorly understood. Using metabolomics, biophysical, and biochemical approaches, we characterized, in vitro, the metabolic shift of differentiating cardiomyocytes and its implications for oxidative damage. We found that differentiating cardiomyocytes undergo a broad metabolic reprogramming from a glycolytic to an oxidative state, marked by increased activity in key pathways, including malate-aspartate shuttle, glutathione metabolism, and tricarboxylic acid cycle. This metabolic transition was associated with mitochondrial enlargement and increased reactive oxygen species (ROS) production. Intriguingly, despite ROS increase, differentiated cells maintained similar levels of DNA damage as cardiomyoblasts and were more resistant to a H₂O₂ challenge. Our findings suggest that metabolic adaptations during cardiomyocyte differentiation enhance their capacity to mitigate oxidative stress damage, providing an adaptive avenue that enables cardiomyocyte survival upon exposure to an oxygen-rich environment.