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<p>The Circle of Willis perforation (cWp) mouse model is widely used in subarachnoid hemorrhage (SAH) research but involves sacrificing the external carotid artery (ECA), which may have a potential affect on the hemodynamic in the carotid arteries and cortical perfusion. We propose a modified approach using needle puncture via the common carotid artery (CCA) to preserve carotid vascular integrity. Twenty-seven C57BL/6 mice were randomly assigned into three groups and underwent cWp surgery in three procedures: sham (<i>n</i> = 3), ECA (<i>n</i> = 12), and CCA (<i>n</i> = 12). Surgical duration, success rate, intraoperative intracranial pressure (ICP) fluctuations, 24-hour mortality, and neurological deficits were assessed. The CCA approach achieved a 100% success rate and shorter surgical duration than the ECA approach (ECA 73 ± 18 vs. CCA 36 ± 10 min, <i>P</i> &lt; 0.05). ICP fluctuations and mortality rates were comparable between the ECA and CCA groups (<i>P</i> &gt; 0.05), indicating that the CCA approach shared a similar pattern with the ECA approach. Neurological outcomes were similar across SAH groups (CCA SAH induction and ECA SAH induction) but worse than sham (<i>P</i> &lt; 0.05) in terms of body weight loss, open-filed test and Rotarod test performances. This modified cWp CCA approach, which preserves the carotid structures, helps eliminate hemodynamic bias and offers a potentially more efficient alternative with a shorter surgical duration compared to the classical ECA approach. It may prove to be a valuable option for broader application in SAH preclinical research.</p>

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A modified surgical approach to induce circle Willis perforation in mice using the common carotid artery

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月21日

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  • Rui Zhang,
  • Dilaware Khan,
  • Sajjad Muhammad

摘要

The Circle of Willis perforation (cWp) mouse model is widely used in subarachnoid hemorrhage (SAH) research but involves sacrificing the external carotid artery (ECA), which may have a potential affect on the hemodynamic in the carotid arteries and cortical perfusion. We propose a modified approach using needle puncture via the common carotid artery (CCA) to preserve carotid vascular integrity. Twenty-seven C57BL/6 mice were randomly assigned into three groups and underwent cWp surgery in three procedures: sham (n = 3), ECA (n = 12), and CCA (n = 12). Surgical duration, success rate, intraoperative intracranial pressure (ICP) fluctuations, 24-hour mortality, and neurological deficits were assessed. The CCA approach achieved a 100% success rate and shorter surgical duration than the ECA approach (ECA 73 ± 18 vs. CCA 36 ± 10 min, P < 0.05). ICP fluctuations and mortality rates were comparable between the ECA and CCA groups (P > 0.05), indicating that the CCA approach shared a similar pattern with the ECA approach. Neurological outcomes were similar across SAH groups (CCA SAH induction and ECA SAH induction) but worse than sham (P < 0.05) in terms of body weight loss, open-filed test and Rotarod test performances. This modified cWp CCA approach, which preserves the carotid structures, helps eliminate hemodynamic bias and offers a potentially more efficient alternative with a shorter surgical duration compared to the classical ECA approach. It may prove to be a valuable option for broader application in SAH preclinical research.